The Cell Division Cycle 73(Cdc73)Deletion Mutant Inhibits Sexual Reproduction and Mitosis of Fission Yeast Cells
10.13865/j.cnki.cjbmb.2024.04.1481
- VernacularTitle:细胞分裂周期蛋白73基因缺失抑制粟酒裂殖酵母细胞的有性生殖和有丝分裂
- Author:
Meng-Nan LIU
1
;
Xin BAI
;
Wen YU
;
Xin-Lin LI
;
Xiang DING
;
Yi-Ling HOU
Author Information
1. 西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充 637009
- Keywords:
cell division cycle 73(cdc73)gene;
Schizosaccharomyces pombe;
sexual reproduction;
mi-tosis;
cell dynamics
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(6):807-818
- CountryChina
- Language:Chinese
-
Abstract:
The cdc73(cell division cycle 73)gene encodes the RNA polymerase Ⅱ cofactor Cdc73 in fis-sion yeast(Schizosaccharomyces Pombe),and is involved in G2 checkpoint activation and regulates the cell cycle.However,whether Cdc73 regulates cell mitotic dynamics is unknown.In this study,fluores-cent protein labeling and live cell imaging techniques were used to investigate the effects of cdc73 deletion on sexual reproduction and the dynamics of microtubules,actin,mitochondria,and histones during mito-sis.The results showed that in sexual reproduction,cdc73 deletion resulted in a 14.23％increase in the length of ascospores and a 64.08％decrease in the number of cells producing four spores.Analysis of the live cell imaging results revealed that,in mitosis,the elongation length of microtubules in anaphase was shortened by 11.21％,and the elongation time was reduced by 17.39％;the formation and contraction rates of actin rings decreased by 33.33％and 26.09％,respectively,and the formation and contraction times were prolonged by 58.00％and 40.38％,respectively.Meanwhile,the expression levels of actin ring,mitochondrion,and histones also increased.This study revealed the cdc73 deletion inhibits spindle elongation and delays actin ring formation and contraction in mitosis,which provides some scientific basis for further exploring the involvement of Cdc73 in regulating microtubule and actin dynamics in cell divi-sion.
