Phenotypic and molecular characteristics of a Salmonella Grumpensis isolate from a patient with diarrhea in Shanghai,China
10.3969/j.issn.1002-2694.2024.00.115
- VernacularTitle:上海市首次自腹泻患者中检出格隆柏沙门菌的表型和分子特征研究
- Author:
Wen-Qing WANG
1
;
Wei-Chun HUANG
;
Jing-Hua SU
;
Shu-Qi YOU
;
Ying-Jie ZHENG
;
Bo-Wen YANG
;
Hong HUANG
;
Li-Peng HAO
;
Xue-Bin XU
Author Information
1. 上海市浦东新区疾病预防控制中心微生物检验科,上海 200136;复旦大学浦东预防医学研究院,上海 200136
- Keywords:
Salmonella Grumpensis;
whole genome sequencing;
multilocus sequence typing;
antimicrobial resistance gene;
virulence gene
- From:
Chinese Journal of Zoonoses
2024;40(8):732-738
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at studying the phenotypic and molecular characteristics of a Salmonella Grumpensis isolate from a patient with diarrhea in Shanghai,to provide evi-dence for the prevention of salmonellosis.Biochemical identifi-cation,serum agglutination testing,antimicrobial susceptibility testing,and whole genome sequencing(WGS)were performed on isolate 2023JD76.Global Salmonella Grumpensis genome sequences were searched and downloaded for serotyping predic-tion,multilocus sequence typing(MLST),prediction of anti-microbia resistance genes and virulence genes,and phylogenetic analysis of 2023JD76.The 2023JD76 strain was identified as Salmonella Grumpensis(13,23:d:1,7)with ST2060,and was susceptible to 20 antimicrobial agents.Strain 2023JD76 carried the aminoglycoside resistance gene aac(6')-Iaa and five types of virulence genes:the adhesion genes csg and rat;the secretion and transport genes sip and inv;the typhoid toxin genes cdt and plt;the invasive gene nutrient metabolism factor mgt;and the antimicrobial peptide resistance factor mig.Global S.Grumpensis strains harbored ten types of antimicrobial resistance genes whose prevalence ranged from 58.33％to 100％.The global genome sequences of S.Grumpensis were divided into two lineages.Lineage I was dominated by ST751(88.89％,16/18),and lineage Ⅱ was dominated by ST2060(89.47％,17/19).The genome sequence of strain 2023JD76 belonged to lineage Ⅱ,and was closely related to the genome sequences from human fecal and human cerebrospinal fluid.This study provides the first report of a S.Grumpensis isolate from the stool of a patient with diarrhea in China.Considerable variability in antimicrobial resistance genes was observed among genome sequences from different sources,and the strains harbored a substantial number of virulence genes.Enhanced surveillance should be emphasized to prevent a potential risk of global dissemination.
