Comparative proteomics analysis of human villous trophoblasts during the infection of Listeria monocytogenes
10.3969/j.issn.1002-2694.2024.00.100
- VernacularTitle:单增李斯特菌感染人绒毛膜滋养层细胞比较蛋白质组学研究
- Author:
Yun-Yi ZHANG
1
;
Zheng ZHANG
;
Jun-Yan ZHANG
;
Hong-Hu CHEN
;
Li ZHAN
;
Jian-Cai CHEN
Author Information
1. 浙江省疾病预防控制中心,杭州 310051
- Keywords:
Listeria monocytogenes;
human villous trophoblasts HTR-8/Svneo;
proteomics;
TMT proteomics technique
- From:
Chinese Journal of Zoonoses
2024;40(7):628-635
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at analyzing the changes in the cell proteome of HTR-8/Svneo human villous trophoblasts during infection with Listeria monocytogenes,to explore the mechanisms involved in L.monocytogenes infection of the placenta at the molecular level in host cells.Tandem Mass Tag(TMT)proteomics was used to compare the quantitative proteome be-tween the HTR-8/Svneo infection group and control group.The Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases were used to analyze enrichment in the molecular functions,biological processes,and metabolic pathways of differentially expressed proteins.A total of 76 285 peptides and 6 979 quantifiable proteins were identified.A total of 356 proteins showed significant differential expression:153 up-regulated and 203 down-regulated.The protein with the high-est fold change was microtubule motor protein(B4DYE2),and the protein with the lowest fold change was translation initia-tion factor 1(Q6IAV3).GO functional enrichment and KEGG metabolic pathway enrichment analyses indicated that the up-regulated proteins were involved primarily in biological processes and metabolic pathways such as the movement of microtubules and microfilament as well as autophagy,whereas the down-regulated proteins were involved mainly in primary metabolic pathways,such as carbon metabolism,nitrogen metabolism,amino acid biosynthesis,and nucleic acid metabolism,as well as ubiquitin mediated protein hydrolysis.In the process of infection of HTR-8/Svneo by L.monocytogenes,the basal metabolism of host cells may be significantly diminished,and the autophagy level may be high.The significant up-regulation of cell migration-inducing and hyaluronan-binding protein CEMIP(Q8WUJ3)suggested an important role of this protein in regulating the migration and invasion of trophoblast cells,thus causing adverse pregnancy outcomes.Our findings may provide new ideas for the study of the molecular mechanism of L.monocytogenes infection of the placenta.
