Acupoint application of seed of Chinese dodder at Sanyinjiao acupoint improves pregnancy outcome and Th1/Th2 balance at maternal-fetal interface in recurrent abortion rat
10.3969/j.issn.1000-484X.2024.12.021
- VernacularTitle:菟丝子三阴交穴位贴敷改善复发性流产大鼠妊娠结局及母胎界面Th1/Th2平衡
- Author:
Xuemei CHEN
1
;
Zhengyan ZHU
Author Information
1. 武汉市第三医院妇科,武汉 430074
- Keywords:
Seed of Chinese dodder;
Sanyinjiao acupoint;
Acupoint application;
Recurrent abortion;
Pregnancy outcome;
Th1/Th2 balance
- From:
Chinese Journal of Immunology
2024;40(12):2585-2590
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the improvement effect of acupoint application of seed of Chinese dodder at Sanyinjiao acu-point on pregnancy outcome in rats with recurrent abortion,and its influence on the balance of Th1/Th2 at maternal-fetal interface.Methods:SD female pregnant rats were randomly divided into normal pregnant rats group,abortion model group,acupoint application of seed of Chinese dodder group,gavage of seed of Chinese dodder group,acupoint application of starch group,with 15 rats in each group.Except for normal pregnant rats group,pregnant rats of other groups were injected subcutaneously with 0.3 mg/kg bromocriptine on 6～8 days of gestation,for once a day,the abortion model was established,the treatment was given from the first day of pregnancy,and the blood was taken from the abdominal aorta on the 12th day of pregnancy to detect levels of serum prolactin(PRL)and progester-one(P);pregnant rats were sacrificed,the uterus was taken out,the embryo abortion rate was calculated and the uterus weight was weighed to evaluate changes of pregnancy outcome of pregnant rats;the decidua tissue was taken,and the positive expression of pro-gesterone receptor(PR)was detected by immunohistochemical method;Th1 type(TNF-α,IFN-γ)/Th2 type(IL-4,IL-10)cytokines mRNA and protein expressions in decidua tissue were detected by RT-PCR and Western blot.Results:Compared with normal pregnant rats group,abortion model group had an increased embryo abortion rate,and decreased uterine weight,progesterone PRL,P and PR expressions,the decidual tissue Th1 type(TNF-α,IFN-γ)cytokines mRNA and protein expressions were increased,while Th2 type(IL-4,IL-10)cytokines mRNA and protein expressions were decreased(P<0.05).Acupoint application of seed of Chinese dodder and gavage of seed of Chinese dodder could reduce the abortion rate,increase expressions of PRL,P and PR,and correct the Th1/Th2 imbalance,and the therapeutic effect of acupoint application of seed of Chinese dodder is better than that of gavage of seed of Chinese dodder(P<0.05).Conclusion:Application of the seed of Chinese dodder at Sanyinjiao acupoint can improve the adverse pregnancy outcome of abortion model rats,correct Th1/Th2 imbalance,and reduce the abortion rate,and its effect is better than gavage of seed of Chinese dodder.
