Analysis of reconstitution and origin of mast cells and basophils after bone marrow transplantation with flow cytometry
10.3969/j.issn.1000-484X.2024.11.006
- VernacularTitle:流式细胞术分析小鼠骨髓移植后肥大细胞和嗜碱性粒细胞重建及细胞来源
- Author:
Ran GUO
1
;
Wenzhi GUO
Author Information
1. 郑州大学第一附属医院,河南省消化器官移植重点实验室,郑州 450052
- Keywords:
Bone marrow transplantation;
Immune reconstitution;
Mast cells;
Basophils;
Flow cytometry
- From:
Chinese Journal of Immunology
2024;40(11):2273-2278
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the reconstitution and origin of mast cells and basophils in mice after bone marrow trans-plantation for 8 weeks.Methods:Total 5×106 whole bone marrow mononuclear cells(wBMMNC)from C57BL/6 homogeneic mice(CD45.1)were injected into the tail vein of recipient mice(CD45.2)which were received lethal irradiation.Eight weeks after trans-plantation,peripheral blood,peritoneal lavage fluid,spleen and bone marrow of control and transplantation groups were collected.Cell suspension were prepared and red blood cells were lysed,and the total number of nucleated cells were counted.Flow cytometry antibodies were used for staining,and the proportion and number of mast cells and basophils in different tissues were analyzed.Simul-taneously,the cell origin was identified by analyzing the proportion of CD45.1 and CD45.2.Results:After 8 weeks of bone marrow transplantation,the proportion of mast cells in peritoneal cavity and basophils in bone marrow returned to the normal level,and there was no significant with control.The proportions of basophils in peripheral blood and spleen were also reconstituted,but it was different with control group(P<0.05).The percentage of CD45.2+mast cells in peritoneal was about 93.4%,and the percentage of CD45.1+ba-sophils cells in peripheral blood,spleen and bone marrow were 87.6%,96.4%and 90.8%,respectively.Conclusion:After 8 weeks of bone marrow transplantation,mast cells in peritoneal cavity and basophils in bone marrow have been reconstituted quickly,while basophils in peripheral blood and spleen reconstituted relatively slowly.Meanwhile,mast cells differentiate from host resident cells and basophils differentiate from donor bone marrow cells.
