Single cell sequencing analyzes T cell receptor gene differences of patients with viral pneumonia
10.3969/j.issn.1000-484X.2024.04.021
- VernacularTitle:单细胞测序分析病毒性肺炎患者T细胞受体基因差异
- Author:
Enze MA
1
;
Ke NING
;
Xiao ZHU
Author Information
1. 哈尔滨师范大学计算机科学与信息工程学院,哈尔滨 150025
- Keywords:
Single cell sequencing;
Viral pneumonia;
TCR;
Gene
- From:
Chinese Journal of Immunology
2024;40(4):796-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze differences in peripheral blood T-cell receptor(TCR)genes in patients with viral pneumo-nia by single cell sequencing,and to explore its possible pathogenesis.Methods:Data in NCBI database was obtained,and case and control groups were set up for experiments.Single cell sequencing data was analyzed of by R language in RStudio software,tables and images were plotted,and finally conclusions were drawn.Results:There was a significant difference in TCRs between case and healthy control groups.Frequency of use of V and J gene fragments of TCR α and β chains were analyzed,in which frequency of use of TRAV1-2,TRAV29/DV5,TRAJ33,TRAJ48,TRBV20-1,TRBV2,TRBJ2-3 and TRBJ1-1 genes were significantly higher in case group than in control group(P<0.05).Further V-J gene combination analysis showed that the most frequent use of α-chain V-J pairs in case group was TRAV1-2-J33,most frequent use of β-chain V-J pairs was TRBV20-1-J2-1,most frequent use of αβ double-chain V-J pairs was TRAV30-J24-TRBV3-1-J2-7.Conclusion:Peripheral blood TCR genes are significantly altered in patients with viral pneu-monia,which may be related to immunopathogenesis of virus.
