Production of Interleukin-5, Interleukin-13 and Interferon-gamma in Peripheral Blood CD8+T Cells from Children with Wheezing.
- Author:
Moon Hee LEE
1
;
Sena MOON
;
Ji Hyun JANG
;
Myung Sook CHUNG
;
Eugene KIM
;
Jong Seo YOON
;
Hyun Hee KIM
;
Joon Sung LEE
Author Information
1. Department of Pediatrics, College of Medicine The Catholic University of Korea, Seoul, Korea. pedjsyoon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
CD8+ T cell;
Interleukin-5;
Interleukin-13;
Interferon-gamma
- MeSH:
Cell Culture Techniques;
Child;
Humans;
Interferon-gamma;
Interferons;
Interleukin-13;
Interleukin-5;
Interleukins;
Respiratory Sounds;
T-Lymphocytes
- From:Pediatric Allergy and Respiratory Disease
2008;18(3):228-235
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Our objective was to investigate the role of CD8+T cells in pathogenesis of wheezing in children with atopic nature. METHODS: Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8+ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-gamma in the cell culture supernatants. RESULTS: In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-gamma concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. CONCLUSION: The results of this study suggest that CD8+ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5.
