Effects of propranolol on the biological behavior of human umbilical vein endothelial cells and the expression of SOX18,MMP-7,and VEGFA
10.7499/j.issn.1008-8830.2407088
- VernacularTitle:普萘洛尔对人脐静脉内皮细胞生物学行为及SOX18、MMP-7、VEGFA表达的影响
- Author:
Pei ZHOU
1
;
Si-Qing XIE
;
Li-Li ZHONG
;
Xiao-Fang DING
Author Information
1. 湖南省人民医院/湖南师范大学附属第一医院儿童医学中心儿童呼吸病学湖南省重点实验室,湖南长沙 410005
- Keywords:
Propranolol;
Infantile hemangioma;
Sex-determining region Y-box 18;
Matrix metalloproteinase-7;
Vascular endothelial growth factor A;
Human umbilical vein endothelial cell
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(10):1115-1121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propranolol on the proliferation,apoptosis,migration,and tube formation ability of human umbilical vein endothelial cells(HUVEC),as well as its impact on the expression of sex-determining region Y-box 18(SOX18),matrix metalloproteinase-7(MMP-7),and vascular endothelial growth factor A(VEGFA).Methods HUVEC were treated with different concentrations of propranolol,and cell viability was assessed using the CCK-8 method to determine the optimal concentration and treatment duration.The experiment consisted of a control group and groups treated with different concentrations of propranolol(50,100,150 μmol/L).Apoptosis,migration,and tube formation of HUVEC were observed using flow cytometry,wound healing assays,and tube formation assays.Western blot and real-time quantitative PCR were used to detect the expression levels of SOX18,MMP-7,and VEGFA proteins and mRNA.Results Compared to the control group,the apoptosis rate in the propranolol treatment groups increased significantly(P<0.05),and it rose significantly with increasing drug concentration(P<0.05).The wound healing rate decreased in the propranolol treatment groups,and both the number of tube formation nodes and total tube length were reduced(P<0.05).The expression levels of SOX18,MMP-7,and VEGFA proteins and mRNA were downregulated in the propranolol treatment groups(P<0.05).Conclusions Propranolol can inhibit the proliferation,migration,and tube formation ability of HUVEC and promote cell apoptosis,resulting in decreased expression levels of SOX18,MMP-7,and VEGFA.
