Expression and significance of hypoxia-inducible factor 1a and Bcl-2/adenovirus E1B19kDa-interacting protein 3 in children with traumatic brain injury
10.7499/j.issn.1008-8830.2310067
- VernacularTitle:缺氧诱导因子1α、Bc1-2/腺病毒E1B19kDa相互作用蛋白3在儿童创伤性脑损伤中的表达及意义
- Author:
Lei ZHU
1
;
Xue-Cheng WANG
;
Yan-Yan XU
;
Nan WANG
;
Bing-Xin ZHU
;
Zheng-Wei LI
Author Information
1. 徐州医科大学附属徐州儿童医院重症医学科,江苏徐州 221006
- Keywords:
Traumatic brain injury;
Bcl-2/adenovirus ElB19kDa-interacting protein 3;
Beclin-1;
Hypoxia-inducible factor 1α;
Child
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(4):378-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To dynamically observe the changes in hypoxia-inducible factor 1α(HIF-1α)and Bcl-2/adenovirus E1B19kDa-interacting protein 3(BNIP3)in children with traumatic brain injury(TBI)and evaluate their clinical value in predicting the severity and prognosis of pediatric TBI.Methods A prospective study included 47 children with moderate to severe TBI from January 2021 to July 2023,categorized into moderate(scores 9-12)and severe(scores 3-8)subgroups based on the Glasgow Coma Scale.A control group consisted of 30 children diagnosed and treated for inguinal hernia during the same period,with no underlying diseases.The levels of HIF-1α,BNIP3,autophagy-related protein Beclin-1,and S100B were compared among groups.The predictive value of HIF-1α,BNIP3,Beclin-1,and S100B for the severity and prognosis of TBI was assessed using receiver operating characteristic(ROC)curves.Results Serum levels of HIF-1α,BNIP3,Beclin-1,and S100B in the TBI group were higher than those in the control group(P＜0.05).Among the TBI patients,the severe subgroup had higher levels of HIF-1α,BNIP3,Beclin-1,and S100B than the moderate subgroup(P＜0.05).Correlation analysis showed that the serum levels of HIF-lα,BNIP3,Beclin-1,and S100B were negatively correlated with the Glasgow Coma Scale scores(P＜0.05).After 7 days of treatment,serum levels of HIF-1α,BNIP3,Beclin-1,and S100B in both non-surgical and surgical TBI patients decreased compared to before treatment(P＜0.05).ROC curve analysis indicated that the areas under the curve for predicting severe TBI based on serum levels of HIF-1α,BN1P3,Beclin-1,and S100B were 0.782,0.835,0.872,and 0.880,respectively(P＜0.05),and for predicting poor prognosis of TBI were 0.749,0.775,0.814,and 0.751,respectively(P＜0.05).Conclusions Serum levels of HIF-1α,BNIP3,and Beclin-1 are significantly elevated in children with TBI,and their measurement can aid in the clinical assessment of the severity and prognosis of pediatric TBI.IChinese Journal of Contemporary Pediatrics,2024,26(4):378-384]
