Effects of α1-antitrypsin on motor function in mice with immature brain white matter injury
10.7499/j.issn.1008-8830.2309003
- VernacularTitle:α1-抗胰蛋白酶对未成熟脑白质损伤小鼠运动功能的影响
- Author:
Wen-Dong LI
1
;
Juan SONG
;
Han ZHANG
;
Lu-Xiang YANG
;
Yu-Yang YUE
;
Xin-Ling ZHANG
;
Yong WANG
Author Information
1. 郑州大学第三附属医院小儿内科/河南省小儿脑损伤重点实验室,河南郑州 450052
- Keywords:
Hypoxia-ischemia;
White matter injury;
Alpha-1 antitrypsin;
Catwalk gait analysis;
Mouse
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(2):181-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of α1-antitrypsin(AAT)on motor function in adult mice with immature brain white matter injury.Methods Five-day-old C57BL/6J mice were randomly assigned to the sham surgery group(n=27),hypoxia-ischemia(HI)+ saline group(n=27),and HI+AAT group(n=27).The HI white matter injury mouse model was established using HI methods.The HI+AAT group received intraperitoneal injections of AAT(50 mg/kg)24 hours before HI,immediately after HI,and 72 hours after HI;the HI+saline group received intraperitoneal injections of the same volume of saline at the corresponding time points.Brain T2-weighted magnetic resonance imaging scans were performed at 7 and 55 days after modeling.At 2 months of age,adult mice were evaluated for static,dynamic,and coordination parameters using the Catwalk gait analysis system.Results Compared to the sham surgery group,mice with HI injury showed high signal intensity on brain T2-weighted magnetic resonance imaging at 7 days after modeling,indicating significant white matter injury.The white matter injury persisted at 55 days after modeling.In comparison to the sham surgery group,the HI+saline group exhibited decreased paw print area,maximum contact area,average pressure,maximum pressure,paw print width,average velocity,body velocity,stride length,swing speed,percentage of gait pattern AA,and percentage of inter-limb coordination(left hind paw → left front paw)(P<0.05).The HI+saline group showed increased inter-paw distance,percentage of gait pattern AB,and percentage of phase lag(left front paw → left hind paw)compared to the sham surgery group(P<0.05).In comparison to the HI+saline group,the HI+ AAT group showed increased average velocity,body velocity,stride length,and swing speed(right front paw)(P<0.05).Conclusions The mice with immature brain white matter injury may exhibit significant motor dysfunction in adulthood,while the use of AAT can improve some aspects of their motor function.[Chinese Journal of Contemporary Pediatrics,2024,26(2):181-187]
