Temporal changes of pulmonary surfactant protein D in young rats with acute lung injury induced by lipopolysaccharide
10.3969/j.issn.1008-8830.2005.06.002
- VernacularTitle:肺表面活性物质蛋白-D在脂多糖诱导的急性肺损伤幼鼠中的时序变化
- Author:
SHU LIN-HUA
1
;
WU XIU-QING
;
WEI KE-LUN
;
SHU LIN-HONG
;
XUE XIN-DONG
;
WU HONG-MIN
;
ZONG ZHI-HONG
;
GAO HONG
Author Information
1. 中国医科大学
- Keywords:
Lipopolysaccharide;
Acute lung injury;
Pulmonary surfactant protein D;
Alveolar type Ⅱ cells;
Lamellar body;
Rats
- From:
Chinese Journal of Contemporary Pediatrics
2005;7(6):483-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective Pulmonary surfactant protein-D (SP-D) is regarded as a valuable biomarker in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), but the alterations of SP-D in lung tissues in the early course of ALI remain unknown. This study was designed to explore the temporal fluctuations of SP-D and SP-D mRNA in young rats with ALI induced by lipopolysaccharide (LPS) , as well as the alterations of ultrastructures of alveolar type Ⅱ (ATⅡ) cells. Methods Rat ALI models were established by intraperitoneal injection of LPS (4 mg/kg). The rats were sacrificed at 6, 12, 24, 36, 48 and 72 hrs after LPS injection (8 rats each time point). Western blot and RT-PCR were employed to detect the contents of SP-D and SP-D mRNA in lung tissues. The ultrastructures of AT Ⅱ cells were studied with transmission electron microscopy. Results Both SP-D mRNA and SP-D levels decreased after 12 hrs of LPS administration. The SP-DmRNA level reached a nadir at 24-36 hrs, but the SP-D level was reduced to its nadir by 48 hrs after LPS administration. LPS resulted in the alterations of lamellar bodies (LBs) in size ( multilamellar forms), density (vacuole-like deformity) and number. The alterations of ultrastructures of AT Ⅱ cells were most significant at 48 hrs. The clinical symptoms of ALI rats were most severe at 48 hrs. Conclusions The alterations of the SP-D level were timedependent in the early course of LPS-induced ALI. The lowest level of SP-D occurred at 48 hrs while severe multideformities of AT Ⅱ cells were presented. A decreased level of SP-D in the lungs in the early stage of ALI may be associated with a worse clinical outcome.
