Protective Effect and Mechanism of Danggui Shaoyao Pulvis on Kidney of Diabetic Nephropathy Rats Based on NF-κB Signaling Pathway
10.3870/j.issn.1004-0781.2024.08.005
- VernacularTitle:基于NF-κB信号通路研究当归芍药散对糖尿病肾病大鼠肾脏的保护作用及其机制
- Author:
Zhenzhong XIA
1
;
Enfeng SONG
Author Information
1. 武汉大学人民医院中医科,武汉 430060
- Keywords:
Danggui Shaoyao Pulvis;
Diabetic nephropathy;
NF-κB signal pathway;
Protective effect
- From:
Herald of Medicine
2024;43(8):1205-1211
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the protective effect of Danggui Shaoyao Pulvis on diabetic nephropathy(DN)in rats by examining the NF-κB signaling pathway and its potential mechanism.Methods Healthy male SD rats of SPF grade were selected and induced with an early DN rat model using a dual method(high-fat and high-sugar diet+intraperitoneal injection of Streptozotocin STZ);the rats were randomly divided into the model control group,Danggui Shaoyao Pulvis group,and irbesartan group,while a blank group was set as the control,Each group received the corresponding drugs via gavage for a 4-week intervention period.Various parameters such as body mass,random blood glucose(GLU),24 h water intake,24 h urine volume,24 h urine protein,blood creatinine(Scr),urea nitrogen(BUN),and kidney hypertrophy index(KW/BW)were recorded and measured.Kidney samples were stained with HE and PAS for observation under a light microscope.The serum levels of TNF-ɑ,IL-1β,and IL-6 inflammatory factors were detected using Elisa.The positive expression of NF-κB protein in renal tissues was determined by immunohistochemistry.Results Compared to the blank control group,the model control group exhibited increased kidney volume,KW/BW,GLU,24 h urinary protein quantity,24 h water intake,24 h urine volume,Scr,BUN,serum levels of TNF-ɑ,IL-1β,IL-6,and positive expression of NF-κB in kidney tissue(P<0.05),while body weight decreased(P<0.05).The kidney tissue also showed significant pathological injury.In contrast,the Danggui Shaoyao Pulvis group demonstrated significantly decreased kidney volume,KW/BW,GLU,24 h urinary protein quantity,24 h water intake,24 h urine volume,Scr,serum levels of TNF-ɑ,IL-1β,IL-6,and positive expression of NF-κB in kidney tissue(P<0.05),along with increased body mass(P<0.05).The pathological damage of kidney tissue was alleviated.Conclusions The findings of this study suggest that Danggui Shaoyao Pulvis can effectively reduce the release of TNF-ɑ,IL-1β,and IL-6 inflammatory cytokines in DN rats.This reduction is achieved by down-regulating the expression of NF-κB protein in the inflammatory signaling pathway of kidney tissue.These results indicate that the modulation of NF-κB protein expression may be a crucial mechanism by which Danggui Shaoyao Pulvis exerts its therapeutic effects in treating DN.
