The Role of HOXA6 and FHIT Methylation Levels in Plasma cfDNA in the Diagnosis of Prostate Cancer
10.11969/j.issn.1673-548X.2024.10.028
- VernacularTitle:血浆cfDNA中HOXA6、FHIT甲基化水平在前列腺癌诊断中的作用
- Author:
Dan LI
1
;
Xuedong CHEN
;
Lin TAN
Author Information
1. 323050 丽水市人民医院泌尿外科
- Keywords:
Prostate cancer;
Methylation;
CfDNA;
HOXA6;
FHIT
- From:
Journal of Medical Research
2024;53(10):157-160,177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the methylation levels of HOXA6 and fragile histidine triad gene(FHIT)in circulating free DNA(cfDNA)in plasma and their role in the diagnosis of prostate cancer(PCa).Methods A total of 192 prostate illness patients who visi-ted our hospital from February 2021 to February 2023 were collected as the study subjects,including 112 PCa patients as the disease group and 80 patients with prostatic hyperplasia as the control group.The methylation specific PCR(MSP)method was applied to detect the methylation levels of HOXA6 and FHIT in plasma cfDNA;the relationship between the methylation levels of HOXA6 and FHIT in plasma cfDNA and clinical data was analyzed;multi-factor Logistic was applied to analyze the influencing factors of PCa occurrence;and the di-agnostic value of HOXA6 and FHIT methylation in PCa was evaluated.Results The methylation rates of HOXA6 and FHIT in the plasma cfDNA of the disease group were significantly higher than those of the control group(P＜0.05);the methylation rates of HOXA6 and FHIT in patients with TNM stages Ⅰ-Ⅱ were lower than those in stages Ⅲ-Ⅳ,while patients with lymph node metastasis had higher HOXA6 and FHIT methylation rates than those without lymph node metastasis(P＜0.05);the sensitivity and specificity of the combina-tion of HOXA6 and FHIT methylation in the diagnosis of PCa were 75.89％and 95.00％,respectively,the diagnostic efficacy of the combination of the two was higher than that of HOXA6 and FHIT methylation alone;multivariate Logistic analysis showed that HOXA6 and FHIT methylation were the influencing factors for the occurrence of PCa(P＜0.05).Conclusion The levels of HOXA6 and FHIT meth-ylation in plasma cfDNA of PCa patients are elevated,they could be the influencing factors of PCa,and the combination of the two factors has higher diagnostic efficacy than individual diagnosis,and has certain diagnostic value.
