Effects of LncRNA TCRGV on Migration,Invasion and Lipid Metabolism of Colorectal Cancer Cells and Its Mechanism
10.11969/j.issn.1673-548X.2024.09.016
- VernacularTitle:lncRNA TCRGV对结直肠癌细胞迁移、侵袭、脂代谢功能的影响及其作用机制
- Author:
Boya WANG
1
;
Yidan WANG
;
Lu LI
Author Information
1. 450000 郑州大学;450007 郑州大学附属郑州中心医院消化内科
- Keywords:
Colorectal cancer;
lncRNA TCRGV;
Metastasis;
Lipid metabolism reprograming;
SCD1
- From:
Journal of Medical Research
2024;53(9):80-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of long non-coding RNA(lncRNA)TCRGV on the cell biological behavior and lipid metabolism of colorectal cancer(CRC),along with its potential mechanism.Methods The expression level of lncRNA TCRGV in CRC tissues was analyzed using the GEPIA online database.56 pairs of CRC tissues and paracancer tissues were collected.Normal intes-tinal epithelial cells and CRC cell lines were cultured.The expression level of TCRGV in tissues and cell lines was detected using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).A lentivirus was used to construct a TCRGV overexpression CRC cell model.The effects of TCRGV overexpression on cell migration and invasion capabilities were examined using the Transwell method.The impact of TCRGV on cellular lipid droplet formation was analyzed through Nile red staining.Triglycerides,total cholesterol,and free cholesterol content detection kits were used to detect the content of lipid metabolites in in CRC cells.The expression of the lipid metabo-lism-related protein stearoyl-coenzyme A desaturase 1(SCD1)was detected using RT-qPCR and Western blot to explore its relation-ship with TCRGV.Results The expression level of TCRGV was significantly downregulated in CRC tissues and cells.Compared with the control group,overexpression of TCRGV significantly inhibited the migration and invasion capabilities of CRC cells,significantly de-creased intracellular lipid droplet accumulation,and significantly reduced the contents of total cholesterol,triglycerides,and free choles-terol.The expression level of SCD1 was significantly higher in CRC tissues than that in paracancer tissues.Overexpression of TCRGV sig-nificantly inhibited the expression of SCD1 at both mRNA and protein levels.Conclusion lncRNA TCRGV is downexpressed in CRC and is a potential anticancer molecule.Overexpression of TCRGV may inhibit the cell migration,invasion and lipid reprogramming of CRC by regulating SCD1.
