Role of Intestinal Sympathetic Nerve in the Improvement of Obesity-related Hypertension by Carotid Baroreceptor Stimulation
10.11969/j.issn.1673-548X.2024.08.007
- VernacularTitle:肠道交感神经在颈动脉窦压力感受器电刺激改善肥胖相关性高血压中的作用
- Author:
Lingling XIAO
1
;
Ling SHU
;
Xuesheng FANG
Author Information
1. 430060 武汉大学人民医院心内科、武汉大学心血管病研究所、心血管病湖北省重点实验室
- Keywords:
Obesity-related hypertension;
Sympathetic nervous;
Carotid baroreceptor stimulation;
6-OHDA
- From:
Journal of Medical Research
2024;53(8):28-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of intestinal sympathetic nerve in the improvement of obesity-related hypertension(OH)by carotid baroreceptor stimulation(CBS).Methods Twenty male SD rats were randomly divided into four groups:normal control(C-sham)group,OH-sham group,OH-CBS group and OH-6-OHDA-sham group,with 5 rats in each group.The OH model was in-duced by feeding with high-fat diet and 10％fructose water for 4 weeks.All rats were implanted with CBS stimulator at the end of the 4th week.The OH-CBS group received electrical stimulation,while the OH-sham,C-sham and OH-6-OHDA-sham groups received no stimulation.The OH-6-OHDA-sham group was intraperitoneally injected with 6-OHDA at the 6th week.Blood pressure and body weight of all rats were measured weekly.After 12 weeks,the weight of visceral fat was recorded,and serum glucose and lipid metabolism indexes were detected.Immunofluorescence staining was used to detect tyrosine hydroxylase(TH),mucin-2(MUC2),and zonula oc-cludens-1(ZO-1)in the ileum.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect MUC2,ZO-1 and interleukin-22(IL-22).Results Compared with the C-sham group,the OH-sham group had significant in-creases in blood pressure,the intestinal TH was overexpressed in OH-sham group(P＜0.05),blood pressure,body weight,visceral fat content,serum low-density lipoprotein,glucose,free fatty acid,triglyceride,and total cholesterol were significantly increased(P＜0.05),and serum high-density lipoprotein,IL-22,MUC2,and ZO-1 were decreased(P＜0.05).The TH overexpression was sig-nificantly reduced,the blood pressure,body weight,visceral fat content,serum low-density lipoprotein,glucose,free fatty acid,triglyc-eride,total cholesterol of OH rats were significantly decreased,the expression of serum high-density lipoprotein,IL-22,MUC2 and ZO-1 were also increased in OH-CBS group and OH-6-OHDA-sham group under high-fat and high-sugar feeding.Conclusion Intestinal sympathetic nerve plays an important role in the improvement of OH by CBS.
