Role and Mechanism of UBE2C in the Proliferation of MCF-7 Cells in Breast Cancer
10.11969/j.issn.1673-548X.2024.07.015
- VernacularTitle:UBE2C在乳腺癌MCF-7细胞增殖中的作用及机制研究
- Author:
Qiong WU
1
;
Yongfeng JIA
Author Information
1. 010030 呼和浩特,内蒙古医科大学基础医学院
- Keywords:
Ubiquitin-conjugating enzyme 2C;
Breast cancer;
CyclinD1;
Proliferation
- From:
Journal of Medical Research
2024;53(7):73-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of silencing ubiquitin-conjugating enzyme 2C(UBE2C)on the proliferative capacity of human breast cancer cells MCF-7 and to preliminarily investigate its mechanism of action.Methods Silencing of UBE2C gene in MCF-7 cells using small interfering RNA(si-RNA).CCK-8 assay and clone formation assay were used to detect cell proliferation a-bility.The expression of cell cycle-associated protein kinase inhibitor protein p21 as well as cell cycle-promoting proteins CyclinD1,cyclin-dependent kinase 4(CDK4),and cyclin-dependent kinase 6(CDK6)were detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot at the mRNA and protein levels,respectively.Results Compared with the cells in blank control group and negative control(si-NC)group,the expression of UBE2C was down-regulated in cells of si-UBE2C group.The re-sults of CCK-8 assay and clone formation assay showed that the proliferative ability of cells in si-UBE2C group was significantly de-creased.Compared with the cells in blank control group and si-NC group,the expression of cell cycle-related protein kinase inhibitor protein p21 in the cells of si-UBE2C group was up-regulated at both the mRNA and protein levels,while the expression of cell cycle-promoting proteins CyclinD1,CDK4 and CDK6 was down-regulated at both the mRNA and protein levels.Conclusion UBE2C can reg-ulate the proliferative capacity of MCF-7 cells and promote breast cancer progression by mediating the expression of CyclinD1/CDK4/CDK6 complex and p21.
