Establishment of Lung Cancer Organoid Model and it's Preliminary Drug Sensitivity Test
10.11969/j.issn.1673-548X.2024.06.017
- VernacularTitle:肺癌类器官疾病模型的建立与药敏初步试验
- Author:
Hongwei HUA
1
;
Gang DING
Author Information
1. 201318 上海国际医学中心;201315 上海和睦家新城医院
- Keywords:
Lung cancer;
Three-dimensional culture;
Organoid;
Drug sensitivity test
- From:
Journal of Medical Research
2024;53(6):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method for in vitro culture of organoid model of lung cancer,and use the organoid model for preliminary detection of personalized drug sensitivity.Methods Lung cancer patient tumor tissues were obtained by techniques such as surgery,fiberoptic bronchoscopy and CT-guided lung puncture biopsy.The lesion tissues were washed,cut,digested and filtered to ob-tain lung cancer cells.The lung cancer cells were resuspended in matrigel and inoculated in culture dishes for 3D organoid culture.ki-67 and CD133 immunohistochemistry were used to compare the correlation between organoids and parent tumor tissues.Chemotherapeutic drugs(vinorelbine,cisplatin,paclitaxel,gefitinib and pemetrexed)were used to analyze drug sensitivity results.Results Lung cancer organoids showed obvious nuclear atypia,consistent with parent lung cancer tissues.Lung cancer organoids showed strong positive CD133 immunohistochemistry,suggesting that lung cancer organoids were enriched with tumor stem cells.The proportion of ki-67 positive cells exceeded 50％,indicating that the organoids had certain proliferation ability.After treatment with chemotherapeutic drugs,lung cancer organoids were highly sensitive to cisplatin,gefitinib and paclitaxel,but less sensitive to vinorelbine and pemetrexed.Conclusion This study successfully established an in vitro culture system of lung cancer organoids,which can be preliminarily used for in vitro drug sensi-tivity test.
