Measurement of very low-molecular weight metabolites by traveling wave ion mobility and its use in human urine samples
10.1016/j.jpha.2023.12.011
- Author:
Kurilung ALONGKORN
1
;
Limjiasahapong SUPHITCHA
;
Kaewnarin KHWANTA
;
Wisanpitayakorn PATTIPONG
;
Jariyasopit NARUMOL
;
Wanichthanarak KWANJEERA
;
Sartyoungkul SITANAN
;
Stephen Choong Chee Wong
;
Sathirapongsasuti NUANKANYA
;
Kitiyakara CHAGRIYA
;
Sirivatanauksorn YONGYUT
;
Khoomrung SAKDA
Author Information
1. Siriraj Center of Research Excellent in Metabolomics and Systems Biology(SiCORE-MSB),Faculty of Medicine Siriraj Hospital,Mahidol University,Bangkok,10700,Thailand;Siriraj Metabolomics and Phenomics Center,Faculty of Medicine Siriraj Hospital,Mahidol University,Bangkok,10700,Thailand;Department of Biomedical Informatics,University of Arkansas for Medical Sciences,Little Rock,AR,72205,USA
- Keywords:
Very low-molecular-weight metabolites;
Traveling wave ion mobility;
Collision cross-section;
Human urine
- From:
Journal of Pharmaceutical Analysis
2024;14(5):733-743
- CountryChina
- Language:Chinese
-
Abstract:
The collision cross-sections(CCS)measurement using ion mobility spectrometry(IMS)in combination with mass spectrometry(MS)offers a great opportunity to increase confidence in metabolite identifi-cation.However,owing to the lack of sensitivity and resolution,IMS has an analytical challenge in studying the CCS values of very low-molecular-weight metabolites(VLMs ≤ 250 Da).Here,we describe an analytical method using ultrahigh-performance liquid chromatography(UPLC)coupled to a traveling wave ion mobility-quadrupole-time-of-flight mass spectrometer optimized for the measurement of VLMs in human urine samples.The experimental CCS values,along with mass spectral properties,were reported for the 174 metabolites.The experimental data included the mass-to-charge ratio(m/z),retention time(RT),tandem MS(MS/MS)spectra,and CCS values.Among the studied metabolites,263 traveling wave ion mobility spectrometry(TWIMS)-derived CCS values(TWCCSN2)were reported for the first time,and more than 70％of these were CCS values of VLMs.The TWCCSN2 values were highly repeatable,with inter-day variations of＜1％relative standard deviation(RSD).The developed method revealed excellent TWCCSN2 accuracy with a CCS difference(△CCS)within±2％of the reported drift tube IMS(DTIMS)and TWIMS CCS values.The complexity of the urine matrix did not affect the precision of the method,as evidenced by △CCS within±1.92％.According to the Metabolomics Standards Initiative,55 urinary metabolites were identified with a confidence level of 1.Among these 55 metabolites,53(96％)were VLMs.The larger number of confirmed compounds found in this study was a result of the addition of TWCCSN2 values,which clearly increased metabolite identification confidence.
