Construction and research progress of organoid models for retinitis pigmentosa

VernacularTitle:基于类器官的视网膜色素变性疾病模型构建及研究进展
Author: Linyao XIE ¹ ; Jiansu CHEN ; Yonglong GUO
Author Information

1. 暨南大学基础医学与公共卫生学院，广州　510632
Keywords: Retinal organoids; Retinitis pigmentosa; Disease model
From: Chinese Journal of Experimental Ophthalmology 2024;42(5):473-477
CountryChina
Language:Chinese
Abstract: After more than ten years of development, retinal organoid (RO) based on pluripotent stem cells can highly simulate the development process of human retina, provide insight into the mechanism of retinal development and provide new treatments for retinal diseases.At present, RO has been widely used in the research of the mechanism and treatment of retinal diseases, especially in retinitis pigmentosa (RP).This review summarizes the methods of preparing RO from human pluripotent stem cells, and elaborates the mechanism and therapeutic application of RO-RP disease model in different mutated genes such as PRPF31, RPGR, CRB1, RP2, IMPG2, NR2E3, USH2A, PDE6B and TRNT1, as well as the research progress of RO in drug screening, drug toxicity testing, gene therapy and cell therapy, and discusses the research and application challenges of RO.