Regulation effect of siRNA on β-globin in erythrocytes of hemoglobin H disease
10.3760/cma.j.issn.0253-2727.2021.12.009
- VernacularTitle:siRNA对血红蛋白H病红系细胞β珠蛋白的调控作用
- Author:
Hailing CHENG
1
;
Rongrong LIU
Author Information
1. 广西医科大学第一附属医院血液科，南宁 530021
- Keywords:
Thalassemia;
Hemoglobin H disease;
β-globin;
siRNA
- From:
Chinese Journal of Hematology
2021;42(12):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of targeted siRNA on β-globin in erythroid cells cultured by targeted differentiation in vitro and provide new theoretical support for gene therapy for hemoglobin H (HbH) disease.Methods:Based on the β-globin gene expression results, the optimal siRNA sequence and its effective action dose were screened in erythroid cells, and the effect of the effective dose of the optimal siRNA on the regulation of β-globin expression and apoptosis in erythroid cells was examined. The effective dose of the optimal siRNA was applied to erythroid cells with HbH disease. The effects of transfected siRNAs on red line cells with HbH disease were comprehensively evaluated by measuring the expression of β-globin, reactive oxygen species (ROS) , and apoptosis rates.Results:Within 96 hours after transfection, siRNA2 significantly downregulated β-globin expression in in vitro cultured erythroid cells, but not α-globin. siRNA silencing effect and duration of effect were dose-dependent. siRNA2 downregulated β-globin expression, reduced intracellular ROS production, and decreased apoptosis rate in erythroid cells with HbH disease.Conclusion:Targeted siRNAs can downregulate β-globin expression, reduce intracellular ROS production, and downregulate apoptosis rate in erythroid cells with HbH disease.
