Intervention effects of rhoside in SCI rats by regulating the MAPK/ERK signaling pathway
10.3969/j.issn.1672-2159.2024.05.008
- VernacularTitle:基于NGF/PI3K/TRPV1信号通路探究参苓白术散对腹泻型肠易激综合征小鼠的干预机制
- Author:
Wei ZHU
1
;
Min-Tao JIAN
;
Ting-Ting ZENG
Author Information
1. 410007 湖南中医药大学第一附属医院肛肠科
- Keywords:
Shenling White Surgery Powder;
nerve growth factor/phosphatidylinositol 3-kinase/transient receptor potential vanilloid receptor 1;
diarrhea type irritable bowel syndrome;
inflammatory response
- From:
Modern Interventional Diagnosis and Treatment in Gastroenterology
2024;29(5):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of intervention of phosphatidylinositol 3-kinase/nerve growth factor/vanilloid receptor 1(NGF/PI3K/TRPV 1)signaling pathway.Methods Forty 10 SPF SD male rats were selected,including 10 in blank group,13 in model group,7 in study 1 and 9 in study 2.The control group and model group will only receive saline ingavage without any other treatment.Study group 1 received 20 mg/kg aqueous solution for gavage,and study 2 group gave 4 g/kg for gavage.The Bristol score,the spleen coefficient and thymus coefficient were analyzed,and the expression levels of IgA,IgG,IgM,IL-1 β,IL-6,TNF-α and NGF/PI3K/TRPV 1 protein expression were measured.Results Compared with the blank group,Bristol score,IgA,IgG,IgM,IL-1 β,IL-6,TNF-α were increased(P＜0.05),Bristol score,spleen coefficient,thymus coefficient,IgA,IgG,IgM,IL-1 β,IL-6,TNF-α levels and NGF,PI3K,and TRPV 1 were decreased(P＜0.05).Conclusion Shenling powder inhibited NGF/PI3K/TRPV 1 pathway,improved gastrointestinal function and stool state,reduced inflammation,and improved the homeostasis of immune function,with remarkable intervention effect.
