Expression and biological function of Let-7c-5p in esophageal squamous carcinoma
10.3969/j.issn.1672-2159.2024.03.010
- VernacularTitle:Let-7c-5p在食管鳞癌中的表达及生物学功能
- Author:
Ya-Xin ZHENG
1
;
Ming KANG
Author Information
1. 646000 西南医科大学附属医院消化内科
- Keywords:
let-7c-5p;
esophageal squamous carcinoma;
cell proliferation;
cell migration
- From:
Modern Interventional Diagnosis and Treatment in Gastroenterology
2024;29(3):302-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of let-7c-5p in esophageal squamous carcinoma(ESCC),and also to analyse the effect of let-7c-5p on the proliferation and migration of ESCC cells KYSE150.Methods Forty-two cases of esophageal squamous carcinoma(ESCC)were collected from our hospital,and the paracancerous tissues were used as the control group.The expression of let-7c-5p in ESCC tissues and their adjacent paracancerous tissues was determined by qRT-PCR(real-time fluorescence quantitative polymerase chain reaction);KYSE150 cells were transfected with liposomal Lipofectamine 2000,and the expression of let-7c-5p in transfected KYSE150 cells was determined by qRT-PCR;and the proliferation of the transfected cells was measured by CCK-8 technique;the migration activity of the cells was measured by wound healing assay and Transwell migration assay.Results The expression of let-7c-5p mRNA in ESCC tissues was significantly lower than that in normal tissues next to their cancer(P=0.000);after transfection of KYSE150 cells,in terms of expression,let-7c-5p mimics were significantly higher than that in the NC group(P＜005);the proliferative function of KYSE150 in the transfected group showed a drastic decrease,and its migratory activity was substantially suppressed.Conclusion let-7c-5p is lowly expressed in esophageal squamous carcinoma,and up-regulation of its expression can significantly inhibit the proliferation and migration of esophageal squamous carcinoma KYSE150 cells,providing a new potential target for the diagnosis and treatment of esophageal squamous carcinoma patients.
