Research advances on the development and application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein system

VernacularTitle:成簇规律间隔短回文重复序列及其相关蛋白基因系统的发展及应用研究进展
Author: Jingjie TAN ¹ ; Yizhi PENG ; Guangtao HUANG
Author Information

1. 遵义医科大学附属医院烧伤整形外科　563003
Keywords: Genes; Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein system; Gene editing; Derived technologies
From: Chinese Journal of Burns 2021;37(7):681-687
CountryChina
Language:Chinese
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein system, as an emerging gene editing system, can be divided into class 1 and class 2 systems according to the number of Cas protein. The CRISPR/Cas9 in class 2 system can cleave target nucleic acid only with the help of Cas9 protein and single-stranded guide RNA, which is currently the most widely used CRISPR/Cas system. In addition to gene editing in the treatment of genetic diseases, a variety of CRISPR/Cas system derived technologies have vast application prospect in the fields of disease-related gene screening, gene expression regulation, and rapid detection, prevention, and control of pathogens. This article summarizes the discovery process of CRISPR/Cas system and applications of several major CRISPR/Cas derived technologies, aiming to provide a reference for researchers in the field of life science.