The Correlation between Changes in Brain Gut Peptides and Pharyngeal Reflux In Sleep Deprived Rats
10.3969/j.issn.1006-7299.2024.06.012
- VernacularTitle:睡眠剥夺大鼠脑肠肽改变在大鼠咽喉反流中的作用
- Author:
Xue ZHANG
1
;
Mei HAO
;
Yanping ZHANG
Author Information
1. 解放军总医院第六医学中心耳鼻咽喉科(北京 100091)
- Keywords:
Improved multi-platform water environment method;
Sleep deprivation;
Brain gut peptides;
Laryngopharyngeal reflux
- From:
Journal of Audiology and Speech Pathology
2024;32(6):532-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of changes of brain gut peptides in the serum of sleep deprived rats in the pathogenesis of laryngopharyngeal reflux.Methods Forty healthy adult male SD rats were selected and divided into the experimental group and the control group.The control group slept normally.The experimental group was divided into three subgroups,each with 10 rats.The improved multi-platform water environment method was used to deprive 8 hours of daytime sleep for 1,2,and 3 months,respectively.Detection of motilin(MTL)and somatostatin(SS)concentrations in peripheral blood was completed using ELISA,and detection of gastrin(GAS)concentrations in peripheral blood was conducted using radioimmunoassay.The differences among the groups were compared.Results The GAS concentration in the 2-month group was significantly lower than that in the control group,while the GAS concentration in the 3-month group was significantly higher than that in the 1-month and 2-month groups(P＜0.05).There was no statistically significant difference between the MTL concentration in the three experimental groups and the control group pairwise(P＞0.05),but the MTL concentration in the 2-month group was significantly higher than that in the 3-month group(P＜0.01).There was no statistical difference be-tween the SS concentration in the three experimental groups and the control group,as well as among the three ex-perimental groups.Conclusion Sleep deprivation may be associated with the occurrence of laryngopharyngeal reflux by affecting the secretion of GAS and MTL.
