Role of NID1 in angiogenesis of clear cell renal cell carcinoma
- VernacularTitle:NID1在肾透明细胞癌血管生成中的作用研究
- Author:
Chuangxin SUN
1
;
Gang LI
Author Information
- Keywords: carcinoma,renal cell; extracellular matrix; cell proliferation; angiogenesis
- From: Tianjin Medical Journal 2024;52(10):1009-1013
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the expression of nestin-1(NID1)in clear cell renal cell carcinoma(ccRCC)and its effect on angiogenesis.Methods The expression of NID1 in ccRCC was analyzed using data from the TCGA database,cell lines and clinical samples.Additionally,the relationship between NID1 and the angiogenesis marker CD31 was investigated.A stable knockdown 786-O cell line with reduced NID1 expression was generated through lentiviral transfection.The conditioned medium obtained from these cells was used to treat human umbilical vein endothelial cells(HUVECs).HUVEC cells were divided into four groups:the DMEM group,the blank group,the NID1 empty vector group and the NID1 knockdown group.Cell proliferation ability was assessed using CCK-8 method,while tube formation ability and migration ability were evaluated through tube formation test and scratch test respectively.A mouse model of dextran hydrogel unloaded/NID1 knockdown tumor cell complex culture system was established and divided into the unloaded NID1 group and the NID1 knockdown group.Immunohistochemical staining was performed to detect the expression of CD31.Results Bioinformatics analysis revealed an increased expression of NID1 in ccRCC tissue.Furthermore,500 differentially expressed genes positively correlated with elevated levels of NID1 were primarily enriched in angiogenesis-related processes.The results showed a positive correlation between the expression of NID1 and CD31 in cancer tissue.After treatment with conditioned medium derived from the knockdown group,HUVEC cells exhibited weakened migration and tube formation abilities in the empty vector control group(P＜0.05),while their proliferation ability remained unchanges compared to the empty vector control group(P＞0.05).In vivo experiments using a nude mouse model,the expression of CD31 demonstrated significantly lower levels in the NID1 knockdown group(P＜0.05).Conclusion The expression of NID1 is increased in ccRCC,and it can promote angiogenesis.
