Protective Effect of Irbesartan in Diabetic Nephropathy Rats Through Regulating NLRP3
10.3870/j.issn.1672-0741.23.05.014
- VernacularTitle:厄贝沙坦通过调节NLRP3表达在糖尿病肾病大鼠中的保护作用
- Author:
Qing ZHAO
1
;
Dan WEN
;
Jian YE
Author Information
1. 南昌大学第一附属医院肾内科,南昌 330006;南昌大学肾脏病分子免疫研究所,南昌 330006
- Keywords:
diabetic nephropathy;
NLRP3 inflammasome;
angiotensin receptor blocker;
renal fibrosis;
innate im-munity
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(3):308-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of NLRP3(NOD-like receptor protein 3)in diabetes nephropathy rats,and observe changes in expression of inflammatory and fibrosis factors after NLRP3 silencing and irbesartan(ARB)intervention,so as to explore the protective effect of irbesartan on diabetic nephropathy rats.Methods Male SD rats were randomly divided into 5 groups:Control group,Model group,Model+Blank group,Model+NLRP3 Sh group and Model+ARB group.The rats were killed 8 weeks and 12 weeks after the establishment of diabetes nephropathy model,and the kidney tissues were collected.Real-time PCR was used to detect the mRNA expression of NLRP3,Caspase-1,P65 and Vimentin.Western blot was used to detect the protein expression of NLRP3,P65,HSP47 and Vimentin.Immunofluorescence was used to observe the expression of NL-RP3,Caspase-1 and Vimentin.PAS,PASM and Masson staining were used to observe the pathological changes of kid-ney.Results Compared with Control group,the mRNA expressions of NLRP3,Caspase-1,P65 and Vimentin were increased(all P＜0.05),and the protein expressions of NLRP3,Caspase-1,P65,HSP47 and Vimentin were increased in Model group and Model+Blank group(all P＜0.05).Compared with Model group and Model+Blank group,the above indicators were decreased in Model+NLRP3 Sh group and Model+ARB group(all P＜0.05).And the differences of above indicators between Model group and Model+Blank group were statistically insignificant.The pathological staining results showed that NLRP3 silencing and ARB intervention inhibited the renal damage of diabetic rats,and relieved the glomerular hypertrophy,glomerular sclerosis,basement membrane expansion,and interstitial fi-brosis.Conclusion NLRP3 signaling pathway is activated in the kidney of diabetes nephropathy rats,and the expression of inflammatory and fibrosis factors is upregulated.Irbesartan can block NLRP3 signaling pathway and alleviate renal damage.Innate immune receptor NLRP3 may become a new therapeutic target for diabetes nephropathy.
