Application of FNA combined with next-generation sequencing in the diagnosis and treatment of thyroid nodules
10.3969/j.issn.1006-5725.2024.17.020
- VernacularTitle:细针穿刺活检联合高通量测序技术在甲状腺结节诊疗中的应用
- Author:
Feng ZHU
1
;
Qing LI
;
Xi CHEN
;
Yang HE
;
Lei PENG
Author Information
1. 苏州大学附属第三医院病理科(江苏常州 213003)
- Keywords:
fine needle aspiration;
next-generation sequencing;
gene mutation;
thyroid cancer
- From:
The Journal of Practical Medicine
2024;40(17):2471-2476
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and clinical significance of fine-needle aspiration combined with next-generation sequencing 18 genes detection in the diagnosis of thyroid cancer.Methods The samples were tested by liquid-based cytology and next-generation sequencing.Cytological diagnosis was based on the 3rd edition Bethesda system for reporting thyroid cytopathology.Histological diagnosis was based on the fifth WHO classification criteria for thyroid neoplasms.Results Among 97 thyroid specimens,8 cases(8.25%)were unsatis-fied,44 cases(45.36%)had benign lesions,9 cases(9.28%)had atypical cells of unknown significance,4 cases(4.12%)had suspected follicular or oncocytic tumors.Suspected papillary carcinoma was found in 10 cases(10.31%)and papillary carcinoma in 22 cases(22.68%).A total of 52 cases(53.61%)were mutated,and 10 gene muta-tions were detected,among which BRAF gene mutation had the highest detection rate 63.46%(33/52).BRAF gene mutation was associated with gender,age and cytological diagnosis(P<0.05).Cytology combined with next-generation sequencing improved the diagnostic accuracy(97.0%vs.81.8%),having higher diagnostic efficiency.Conclusion Thyroid fine-needle aspiration cytology combined with next-generation sequencing can promote early diagnosis of thyroid cancer and provide basis for individual treatment of patients.
