Effects of targeted temperature management based on different durations after traumatic brain injury in rats
10.3969/j.issn.1006-5725.2024.12.003
- VernacularTitle:不同时程目标温度管理对颅脑创伤大鼠的脑保护作用
- Author:
Wanyong ZHAO
1
;
Xiaopeng SUN
;
Jingjing WANG
;
Xiaohong LI
;
Hongtao SUN
Author Information
1. 山东大学齐鲁医院德州医院神经外科(山东德州 253014)
- Keywords:
target temperature management;
mild hypothermia;
traumatic brain injury;
time-histories
- From:
The Journal of Practical Medicine
2024;40(12):1625-1630
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of target temperature management based different durations with delayed hypothermia for traumatic brain injury(TBI)in rats.Methods 36 healthy adult male SD rats were randomly divided into NT group,HT4h group,HT24h group and HT48h group.The TBI model of rats was prepared with an electronic controllable cortical injury device.NT group was treated with normal temperature(37℃)4 h after TBI,and the experimental groups were treated with low temperature(33.0±1.0℃)4 h after TBI for 4 h,24 h,and 48 h.Three days after TBI,the motor function of the rats in each group was evaluated by beam walking test and inclined-grid climbing test,EB staining was used to measure the blood-brain barrier perme-ability,the change of hippocampal neurons was observed by Nissl staining,the expression of DCX and GFAP was detected by immunofluorescence,and the expression of Bcl-2 and Bax was measured by Western blot and immuno-histochemistry.Results Compared with NT group,the experimental groups could significantly improve the motor function of TBI rats,reduce the permeability of blood-brain barrier,protect hippocampal neurons,promote DCX expression,inhibit GFAP expression,up-regulate the expression of Bcl-2 protein,and down-regulate the expres-sion of Bax protein.However,the protective effect was more apparent in HT48h group than other experimental groups(P<0.05).Conclusion Long-term delayed target temperature management has a significant brain protective effect.
