The influence of MCP-1/CCR2 axis mediated NF-κB signal pathway on the migration of dental pulp stem cells steamulated by inflamation factor
10.3969/j.issn.1001-3733.2024.05.007
- VernacularTitle:MCP-1/CCR2轴介导NF-κB信号途径对致炎因素刺激的牙髓干细胞迁移的影响
- Author:
Wei LI
1
;
Jian'an PENG
;
Ganjun YANG
;
Hongmei HU
Author Information
1. 343000,井冈山大学医学部口腔系
- Keywords:
MCP-1/CCR2 axis;
Signaling pathway;
Dental pulp stem cells;
Migration
- From:
Journal of Practical Stomatology
2024;40(5):638-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the molecular mechanism of NF-κB signaling pathway mediated by MCP-1/CCR2 axis in the migra-tion of dental pulp stem cells(DPSCs)stemulated by inflamation factor.Methods:DPSCs of SD rats were in vitro cultured,and identified by flow cytometry.The inflammation model of DPSCs was established by LPS treatment,Western blot and Q-PCR were used to detect the expression of MCP-1 and CCR2 in DPSCs of the groups.The inflamatory DPSCs were treated by NF-κB pathway activator TNF-α and NF-κB pathway inhibitor BMS-345541 respectively,the activation of P65 in inflammatory DPSCs was detected by immuno-fluorescence.Scratch experiment was used to detect the migration ability of DPSCs.Results:Higher expression of MCP-1 and CCR2 genes was observed in LPS-induced DPSCs than in the controls,TNF-α treated DPSCs showed higher expression of MCP-1 and CCR2 genes than the LPS tread and the NF-κB pathway inhibitor BMS-345541 treated cells.TNF-α treated DPSCs showed higher scratch healing effect than LPS treated and BMS-345541 treated cells.Conclusion:The expression of MCP-1 and CCR2 genes in DPSCs is higher in inflammatory state,and the activation of NF-κB signaling pathway can enhance this expression,which can ultimately promote the migration ability of DPSCs in inflammatory state.
