Correlation between the Expression of Neutrophil Extracellular Traps Markers cfDNA,CitH3 and MPO-DNA in Patients with T2DM and the Degree of Retinopathy
10.3969/j.issn.1671-7414.2024.06.013
- VernacularTitle:2型糖尿病患者血浆中NETs标志物cfDNA,CitH3和MPO-DNA表达水平与视网膜病变程度相关性研究
- Author:
Yanan WANG
1
;
Xiaonan ZHU
;
Jing WEI
Author Information
1. 河南科技大学第一附属医院眼科,河南 洛阳 471000
- Keywords:
type 2 diabetes mellitus;
diabetic retinopathy;
neutrophil extracellular traps;
circulating free DNA;
citrullinated histone 3;
myeloperoxidase-DNA
- From:
Journal of Modern Laboratory Medicine
2024;39(6):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between the expression levels of neutrophil extracellular traps(NETs)markers circulating free DNA(cfDNA),citrullinated histone 3(CitH3),myeloperoxidase(MPO)-DNA in plasma and the degree of retinopathy in patients with type 2 diabetes mellitus(T2MD).Methods A sum of 80 patients with type 2 diabetes mellitus(T2DM)in First Affiliated Hospital of Henan University of Science and Technology from January to September 2023 were selected as the study objects.According to the retinopathy of T2DM patients,they were divided into non-diabetic retinopathy(NDR)group(n=30),nonproliferative diabetic retinopathy(NPDR)group(n=26)and proliferative diabetic retinopathy(PDR)group(n=24).General data of enrolled patients were collected and plasma cfDNA,CitH3,MPO-DNA levels were detected.Spearman correlation was used to analyze the relationship between cfDNA,CitH3,MPO-DNA and the severity of retinopathy in T2DM patients.ROC curves were used to analyze the efficacy of cfDNA,CitH3,MPO-DNA and their combination in predicting the occurrence of diabetic retinopathy(DR)in T2DM patients.Results The levels of cfDNA(65.13±18.28,83.34±20.10,114.52±32.36 ng/ml),CitH3(17.76±4.24,22.03±5.01,26.06±8.13 ng/ml)and MPO-DNA(0.31±0.04,0.35±0.05,0.45±0.05)in NDR group,NPDR group and PDR group were increased successively,and the differences were statistically significant(F=28.755,13.335,62.470,all P＜0.001).The levels of cfDNA,CitH3 and MPO-DNA in PDR group and NPDR group were higher than those in NDR group(t=7.076,4.837,11.436;3.550,3.455,3.324),and the levels of cfDNA,CitH3,and MPO-DNA in PDR group were higher than those in NPDR group(t=4.127,2.128,7.065),and the differences were statistically significant(all P＜0.05).Spearman correlation analysis showed that cfDNA,CitH3 and MPO-DNA were positively correlated with the severity of retinopathy in T2DM patients(r=0.659,0.470,0.744,all P＜0.001).ROC curves showed that the AUC(95%CI)of cfDNA,CitH3,MPO-DNA and combined detection were 0.846(0.758～0.934),0.776(0.674～0.878),0.847(0.764～0.930)and 0.938(0.889～0.987),respectively,and it had certain predictive value for DR in T2DM patients.Conclusion The levels of NETs markers cfDNA,MPO-DNA and CitH3 in the plasma of DR Patients are increased,and their levels are positively correlated with the degree of diabetic retinopathy,which can predict the occurrence of DR in T2DM patients to a certain extent,and the combined prediction value is the highest.
