Relationship among Serum TRIM22 and KLF2 Levels,Disease Condition and Clinical Outcome of Patients with Active Ulcerative Colitis
10.3969/j.issn.1671-7414.2024.04.026
- VernacularTitle:溃疡性结肠炎活动期患者血清TRIM22和KLF2水平与病情及临床结局的关系
- Author:
Dandan SHEN
1
;
Hong WANG
Author Information
1. 上海健康医学院附属崇明医院消化内科,上海 202150
- Keywords:
tripartite motif protein 22;
Kruppel-like factor 2;
ulcerative colitis
- From:
Journal of Modern Laboratory Medicine
2024;39(4):143-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship among serum tripartite motif protein 22(TRIM22)and Kruppel-like factor 2(KLF2)levels,conditions and clinical outcomes of patients with active ulcerative colitis(UC).Methods A total of 97 patients with active ulcerative colitis admitted to Chongming Hospital Affiliated to Shanghai University of Medical and Health Sciences from January 2020 to January 2023 were selected as active group,56 patients with remission(remission group)and 80 healthy volunteers(control group)as control subjects.The patients in active group were divided into mild group(n=46),moderate group(n=31)and severe group(n=20)according to severity of the disease,which also were divided into improved group(n=68)and non-improved group(n=29)according to the clinical outcome.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum TRIM22 and KLF2 levels.Pearson product moment correlation analysis was used to analyze its association with modified Mayo score.Multi-factorial logistic regression analysis was used to analyze the influencing factors of failure to improve,and the ROC curve was used to assess the predictive value of serum TRIM22 and KLF2 on failure to improve.Results The levels of serum TRIM22(37.16±9.22 pg/ml,51.05±10.83 pg/ml,64.29±13.51pg/ml)in control group,remission group and active group were increased in turn,while the levels of serum KLF2(45.27±7.98 pg/ml,36.91±7.34pg/ml,27.03±6.25 pg/ml)in control group,remission group and active group were decreased in turn,and the differences were statistically significant(F=121.076,143.946,all P＜0.05).The levels of serum TRIM22(56.07±11.18 pg/ml,67.29±13.04pg/ml,78.56±13.69 pg/ml)in mild group,moderate group and severe group were increased in turn,while the levels of serum KLF2(32.07±4.95 pg/ml,25.86±4.32 pg/ml,17.25±4.09 pg/ml)in control group,remission group and active group were decreased in turn,and the differences were statistically significant(F=24.541,74.141,all P＜0.05).Serum TRIM22 was positively associated with modified Mayo score(r=0.692,P＜0.05),while serum KLF2 was negatively associated with modified Mayo score(r=-0.716,P＜0.05)and serum TRIM22(r=-0.659,P＜0.05)in patients with active ulcerative colitis.Compared with mild patients,moderate and severe patients with active ulcerative colitis had an increased risk of failure to improve after clinical treatment(OR=1.232,2.298,all P＜0.05).Elevated TRIM22 level was a risk factor for failure to improve after clinical treatment(OR=1.835,P＜0.05)and elevated KLF2 level was a protective factor(OR=0.731,P＜0.05).Serum TRIM22,KLF2 and two indicators combined had predictive value for failure to improve,with AUC of 0.806,0.803 and 0.907,respectively.The predictive value of the combination indicators was greater than that of single indicator(Z=2.049,2.053,all P＜0.05).Conclusion Serum TRIM22 level was elevated and KLF2 was decreased in patients with active ulcerative colitis,which were related to disease severity and clinical outcome.Combined testing of two indicators can be used as the biochemical marker for early clinical prediction of failure to improve after clinical treatment.
