Lateral fluid percussion injury induces changes of blood-brain barrier permeability in rats

VernacularTitle:侧位液压冲击脑损伤诱发大鼠血脑屏障通透性改变
Author: Zuolin SHI ¹ ; Zhen WANG ; Yushu DONG
Author Information

1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
Keywords: lateral fluid percussion injury(LFPI); cerebral edema; occludin; zonula occludens-1(ZO-1); aqua-porin 4(AQP4); rat
From: Chinese Journal of Neuroanatomy 2024;40(4):492-496
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of lateral hydraulic injury(LFPI)on the integrity of blood-brain bar-rier in rats and its molecular mechanism.Methods:The rat LFPI model was prepared.Neurological impairment of rats was assessed by neurological severity score(NSS).The integrity of blood-brain barrier was assessed by dry/wet ratio test and Evans blue penetration test.The expressions of zonula occludens-1(ZO-1),occludin,and aquaporin 4(AQP4)were detected by Western Blot.Results:LFPI resulted in neurological dysfunction,increased cerebral water content,increased Evans blue permeability,decreased occludin and ZO-1 expression,and increased AQP4 expression.Conclusion:LFPI can cause cerebral edema and damage the integrity of blood-brain barrier in rats.