Establishment of a mouse model to colonize a human commensal Escherichia coli in the early mammalian gut
10.3969/j.issn.1672-8467.2024.06.004
- VernacularTitle:建立人源共生型大肠埃希菌在早期哺乳动物肠道中定植的小鼠模型
- Author:
Dan-Yi LU
1
;
Li-Hui FENG
Author Information
1. 国家儿童医学中心/复旦大学附属儿科医院儿科研究院 上海 201102
- Keywords:
Escherichia coli;
mouse model;
colonization
- From:
Fudan University Journal of Medical Sciences
2024;51(6):900-907,914
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model of human Escherichia coli(E.coli)colonization in early mammalian intestines,and to explore the trend and influencing factors of E.coli colonization in mouse intestines.Methods A wild-type E.coli strain LFYP5,derived from an infant,was inoculated into the SPF mice via gavage.The abundance change curves of E.coli were plotted by collecting intestinal contents or fecal samples from the mice and utilizing plate counting colony forming unit(CFU)methods.This study investigated the effects of different intestinal locations,inoculation ages,weaning time,and cage-changing time on the colonization abundance of E.coli.Additionally,16S rRNA sequencing was used to explore the changes in the microbial composition of the mouse gut after inoculation with E.coli.Results E.coli LFYP5 was able to colonize the intestinal tract of mice before the weaning age.Its abundance decreased rapidly at the weaning stage,independent of the inoculation age,and whether to change the cages.When weaning occurred too early,the abundance of E.coli declined correspondingly earlier.Mice inoculated with E.coli LFYP5 had a lower alpha diversity of the gut microbiota composition and higher relative abundance of the lactobacillus genus compared to mice without E.coli LFYP5 inoculation.Conclusion A mouse model of human E.coli colonization in the early mammalian gut has been successfully established.This model may be used to study the colonization mechanisms of human E.coli in the mammalian gut in the future.
