The flow cytometry analysis of bone marrow plasma cells in patients of different plasma cell dyscrasias
10.3969/j.issn.1672-8467.2024.04.020
- VernacularTitle:不同浆细胞病分型患者骨髓浆细胞的流式特征分析
- Author:
Zhi-Hong JIANG
1
;
Ya-Ting JIANG
;
Xiao-Na WANG
;
Yu-Xin ZHANG
;
Yang-Yang WANG
;
Zheng WEI
Author Information
1. 复旦大学附属中山医院厦门医院血液科 厦门 361015;厦门市恶性肿瘤综合治疗临床研究中心 厦门 361015
- Keywords:
flow cytometry;
bone marrow plasma cell(BMPC);
multiple myeloma(MM);
light-chain amyloidosis(AL);
monoclonal gammopathy of undetermined significance(MGUS)
- From:
Fudan University Journal of Medical Sciences
2024;51(4):602-607,619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differences of characteristic of bone marrow plasma cells in patients of different plasma cell dyscrasias according to International Myeloma Working Group(IMWG)criteria.Methods We analyzed the serological and bone marrow flow cytometry results of patients with plasma cell dyscrasias diagnosed and treated in Department of Hematology,Zhongshan Hospital(Xiamen Branch),Fudan University from Jun 12,2019 to Sep 5,2023 retrospectively.Results A total of 102 patients,63 males and 39 females,aged 22 to 85 years,were included,including 46 patients with monoclonal gammaglobulinemia of unknown significance,39 patients with multiple myeloma,5 patients with smoldering multiple myeloma and 12 patients with light-chain amyloidosis.All patients had M proteinemia,including 58 patients with IgG type and 44 patients with non-IgG type.Plasma cells were detected in the bone marrow of all patients.Clonal plasma cells were detected in the bone marrow of 79 patients.Normal plasma cells were detected in the bone marrow of 63 patients.Both clonal and normal plasma cells were detected in the bone marrow of 40 patients.Clonal plasma cells from bone marrow of 52 patients expressed CD56 and 12 patients expressed CD117.There were no significant differences in gender,age among different disease groups.There were statistical differences in M protein type,the concentration of M protein,serum involved/uninvolved free light chain ratio,the proportion of plasma cells in bone marrow nucleated cells,the proportion of clonal plasma cells in bone marrow nucleated cells,the proportion of clonal plasma cells in all bone marrow plasma cells,and the proportion of normal plasma cells in all bone marrow plasma cells among different disease groups(P<0.05).There was statistical difference in the expression of CD56 in clonal plasma cells among different disease groups(P=0.009),but no statistical difference in the expression of CD117.Conclusion The proportion of clonal plasma cells to all nucleated cells,the proportion of clonal plasma cells to all plasma cells,and the proportion of CD56 expression in abnormal plasma cells in the bone marrow of patients with light amyloidosis were similar to those of monoclonal gammopathy of undetermined significance,but significantly different from those of patients with multiple myeloma.
