Calcium citrate: a new biomaterial that can enhance bone formation in situ
10.3760/cma.j.issn.1008-1275.2012.05.007
- Author:
Li-Ming WANG
1
;
Wei WANG
;
Xiu-Cui LI
;
Lei PENG
;
Zhong-Qin LIN
;
Hua-Zi X(Ü)
Author Information
1. Department of Orthopaedic Surgery,Affiliated Hospital of Binzhou Medical College,Binzhou 256603,Shandong Province,China
- Keywords:
Bone morphogenetic protein-2;
Biocompatible materials;
Calcium citrate;
Gelatin
- From:
Chinese Journal of Traumatology
2012;(5):291-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 (rhBMP-2) on bone regeneration in a bone defect rabbit model.Methods: Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 (CC-rhBMP-2) group and rhBMP-2 only group.Two 10 mm-long and 5 mm-deep bone defects were respectively created in the left and right femoral condyles of the rabbits.Subsequently 5 pellets of calcium citrate (10 mg)combined with rhBMP-2 (2 mg) or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab.Bone granules were obtained at 2,4 and 6 weeks after procedure and received histological analysis.LSD t-test and a subsequent t-test were adopted for statistical analysis.Results: Histomorphometric analysis revealed newly formed bones,and calcium citrate has been absorbed in the treatment group.The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73％±1.26％ vs 48.21％±2.37％ at 2 weeks; 43.40％±1.65％ vs 57.32％±1.47％ at 4 weeks,and 51.32％±7.80％ vs 66.74％±4.05％ at 6 weeks (P＜0.05 for all).At 2 weeks,mature cancellous bone was observed to be already formed in the treatment group.Conclusion: From this study,it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects.This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.
