Therapeutic effect of oroxylin A on rheumatoid arthritis in mice
10.13431/j.cnki.immunol.j.20240039
- VernacularTitle:千层纸素A对类风湿性关节炎小鼠的疗效研究
- Author:
Jingjing YANG
1
;
Jingman LI
;
Jiali WANG
;
Huan DOU
;
Yayi HOU
Author Information
1. 210093,南京大学医学院
- Keywords:
)Oroxylin A;
Rheumatoid arthritis;
Th17;
Treg;
Macrophage
- From:
Immunological Journal
2024;40(3):303-308
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to investigate the therapeutic effect of oroxylin A(OA)on chicken type Ⅱcollagen-induced arthritis(CIA)in mice and observe the changes of immune cells.The CIA model was established,and 40 mg/kg OA was intraperitoneally injected for 10 consecutive days from the 28th day.The mice were sacrificed at three different times during the administration period,and the joints were scored at each time point.HE staining was used to observe the pathology of the mouse ankle joint;flow cytometry was used to detect the changes of Th17,Treg and macrophages in spleen and inguinal lymph nodes;ELISA was employed to detect the expression levels of IL-1β,IL-18 and IL-6 in serum;and qRT-PCR was used to detect the expression levels of IL-1β,IL-18,IL-6 and TNF-β in spleen of mice.Data showed that on the 34th day after OA administration,the joint swelling of CIA mice was significantly relieved,the pathological score was decreased,and the inflammatory cell infiltration was decreased.Flow cytometry results showed that the proportion of Th17 cells and macrophages in the spleen and inguinal lymph nodes of CIA mice in OA group decreased,while the proportion of Treg cells increased.The results of ELISA and qRT-PCR showed that OA could inhibit the level of inflammation in CIA mice.In conclusion,OA can regulate the proportion of immune cells,inhibit the level of inflammation in CIA mice,and then relieve the symptoms of CIA mice.
