Inhibitory effect of flagellin protein combined with rapamycin on 4T1 breast cancer
10.13431/j.cnki.immunol.j.20240006
- VernacularTitle:鞭毛素蛋白联合雷帕霉素对4T1乳腺癌细胞及荷瘤小鼠的抑制作用
- Author:
Li LUO
1
;
Jing ZHANG
;
Zhaozhen ZHUO
;
Xi CHEN
;
Jun YUAN
;
Wei LI
Author Information
1. 550023,贵阳市第二人民医院检验科
- Keywords:
Flagellin;
Rapamycin;
Breast cancer;
Tumor microenvironment
- From:
Immunological Journal
2024;40(1):39-45
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to explore the effect of flagellin+rapamycin on the growth and metastasis of 4T1 breast cancer in tumor-bearing mice,and their regulatory effect on several immunocytes.4T1 cell line was applied to establish an breast cancer model in Balb/c mice,which then injected with flagellin+rapamycin,and the volume and inhibition rate of tumor were recorded.MTT assay was used to detect the inhibitory effect of flagellin+rapamycin against 4T1 cells.Data showed that the combination of flagellin and rapamycin had the best inhibition effect on 4T1 cells,and the same results were found in animal model experiments.Flow cytometry result indicated that flagellin+rapamycin significantly down-regulated the levels of CD11b+Gr-1+myeloid-derived suppressor cells(MDSCs)and CD11b+F4/80+tumor-associated macrophages(TAMs)of tumor-bearing mice.Furthermore,flagellin+rapamycin alleviates the metastasis of 4T1 cancer cells into liver and lung.Taken together,flagellin+rapamycin can inhibit proliferation and metastasis of 4T1 cells,thus exert antitumor effects in mice,which may related with its regulatory effects on immunocytes in tumor microenvironment.
