Effects of Nalbuphine on proliferation,apoptosis and epithelial mesenchymal transformation of colon cancer cells by regulating Hippo/YAP signaling pathway
10.3969/j.issn.1005-6483.2024.10.028
- VernacularTitle:纳布啡调节Hippo/YAP信号通路对结肠癌细胞增殖、凋亡和上皮间质转化的影响
- Author:
Xin ZHAO
1
;
Jian YIN
;
Tong JIA
Author Information
1. 075000 河北省张家口市第五医院麻醉科
- Keywords:
Nalbuphine;
Hippo/YAP signaling pathway;
colon cancer;
proliferation;
apoptosis;
epithelial mesenchymal transformation
- From:
Journal of Clinical Surgery
2024;32(10):1100-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of nalbuphine on the proliferation,apoptosis and epithelial mesenchymal transformation(EMT)of colon cancer(CC)cells by regulating Hippo/YAP signaling pathway.Methods Human colon cancer cell line HCT-116 was cultured in vitro in this study,the activity of HCT-116 cells was first detected by CCK-8 method when the concentration of Nalbuphine was increased,according to the results,the appropriate concentration of Nalbuphine was selected.then,HCT-116 cells were grouped into ctrl group(No other processing is performed),low concentration nalbuphine group(0.10 mmol/L),high concentration nalbuphine group(0.15 mmol/L),and high concentration nalbuphine(0.15 mmol/L)+XMU-MP-1 group(3 μmol/mL Hippo/YAP signal pathway inhibitor).Then,the CCK-8 method was applied to detect the cell proliferation ability of each group;flow cytometry was applied to detect cell apoptosis in each group;Transwell test was applied to detect the ability of cell migration;Western blot was applied to detect the expression of phosphorylated-YAP(p-YAP),YAP,PCNA,BAX,and EMT-related protein E-cadherin,N-cadherin,Vimentin,Snail of cells in each group.Results The results show that compared with the ctrl group,the survival rate of HCT-116 cells,the number of cell migration,the expression of YAP and PCNA,N-cadherin,Vimentin,Snail proteins in the low concentration nalbuphine group and the high concentration nalbuphine group decreased,the cell apoptosis rate,the contents of p-YAP,BAX,and E-cadherin increased(P＜0.05);compared with the high concentration nalbuphine group,the survival rate of HCT-116 cells,the number of cell migration,the expression of YAP and PCNA,N-cadherin,Vimentin,Snail proteins in the high concentration nalbuphine+XMU-MP-1 group increased,the cell apoptosis rate,the contents of p-YAP,BAX,and E-cadherin decreased(P＜0.05).Conclusion It is concluded that Nalbuphine may inhibit the proliferation of CC cells,promote cell apoptosis,and affect the epithelial mesenchymal transformation by activating Hippo/YAP signaling pathway.
