Expression of costimulatory molecules B7H3 and B7H4 in T lymphoblastic lymphoma/leukemia and clinical significance
10.3760/cma.j.issn.0529?5807.2019.05.004
- VernacularTitle:共刺激分子B7H3、B7H4在T淋巴母细胞淋巴瘤/白血病中的表达及其临床意义
- Author:
Guozhen ZHANG
1
;
Jing LI
;
Wenqi BAI
;
Peng BU
;
Huiwen WANG
;
Yanfeng XI
Author Information
1. 山西医科大学基础医学院病理学教研室
- Keywords:
Lymphoma;
Leukemia;
Pathology,clinical
- From:
Chinese Journal of Pathology
2019;48(5):352-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of B7H3 and B7H4 in T lymphoblastic lymphoma/leukemia (T?LBL/ALL) in correlation with clinicopathological parameters and patient prognosis. Methods Immunohistochemistry (IHC) was used to detect the expression of B7H3 and B7H4 protein in 100 cases of T?LBL/ALL(test group) and 30 cases of lymph node reactive hyperplasia (LH) (control group), diagnosed at Shanxi Cancer Hospital from January 2001 to June 2017. Real?time RT?PCR was used to detect the mRNA expression of B7H3 and B7H4 in 50 cases of T?LBL/ALL and 30 cases of LH (control group). Results There were 79 males,21 females. Immunohistochemical results showed that the expression rates of B7H3 and B7H4 were 23%(23/100) and 54%(54/100), respectively. By real?time RT?PCR, the relative expression of B7H3 mRNA in the T?LBL/ALL group was 2.5 times of that of the LH group. The expression levels of B7H4 mRNA in T?LBL/ALL group and LH group were extremely low.Single factor analysis showed that B7H3 protein expression in T?LBL/ALL group was associated with B symptoms and primary nodal disease (P<0.05). B7H4 protein expression was associated with mediastinal broadening and bone marrow involvement (P<0.05). B7H3 protein, B7H3 mRNA, B7H4 protein expression and IPI score were associated with prognosis (P<0.05), and the combined expression of B7H3 and B7H4 was associated with T?LBL/ALL prognosis (P<0.05). Multivariate Cox regression analysis showed that overexpression of B7H3 mRNA was an independent risk factor for the prognosis of patients with T?LBL/ALL (P<0.05). Conclusion Expression of B7H3 and B7H4 is closely corelated with clinicopathological parameters and prognosis of patients with T?LBL/ALL, suggesting that B7H3 and B7H4 expression play an important role in the development of T?LBL/ALL.
