Role of SALL4 in regulating multi-drug resistance of small cell lung cancer and its clinical significance
10.3760/cma.j.issn.0529-5807.2014.09.007
- VernacularTitle:SALL4在调节小细胞肺癌多药耐药中的作用及其临床意义
- Author:
Huanxin LIU
1
;
Yifeng BAI
;
Wei WANG
;
Linlang GUO
Author Information
1. 武警广东总队医院病理科
- Keywords:
Small cell lung carcinoma;
Prognosis
- From:
Chinese Journal of Pathology
2014;(9):604-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of SALL 4 in regulating multi-drug resistance in small cell lung cancer ( SCLC), and to evaluate its clinical significance .Methods The expression of SALL4 protein and gene was detected by Western blot and real-time PCR (RT-PCR) in both H69 and H69AR cell lines, respectively.SALL4 expression in H69AR was blocked by the siRNA, and then the drug-sensitivities of H69AR cell lines to chemotherapeutic drugs such as cisplatin , doxorubicin, and etoposide were evaluated by cell counting kit assay .SALL4 expression was also examined by immunohistochemistry , and correlated with patients′clinicopathological features and prognosis . Results The expression of SALL4 was significantly increased in H69AR cells than in the H69 cells ( P <0.01).Down-regulation of SALL4 increased the drug-sensitivities of H69AR cells to chemotherapeutic drugs (P=0.02).The expression of SALL4 was significantly increased in SCLC than in para-carcinoma tissues ( P<0.01 ) .SALL4 expression correlated with clinical stage , chemosensitivity and overall survival ( P<0.05 ) , but not with patients′age and gender .Conclusion SALL4 is involved in the regulation of multidrug resistance in SCLC;SALL4 may be a potential target gene to evaluate the chemosensitivity and clinical prognosis for SCLC .
