Effect of inhibition of chemokine C-X-C-motif receptor 7 expression on biological properties of human urine-derived stem cells in hypoxia state in vitro
10.16098/j.issn.0529-1356.2024.06.007
- VernacularTitle:抑制趋化因子受体7表达对体外低氧状态下人尿源性干细胞的生物学影响
- Author:
Ji-Shuang TONG
1
;
Chao-Qun HU
;
Yang BI
Author Information
1. 重庆医药高等专科学校附属第一医院实验医学中心,重庆 400014;重庆医科大学附属儿童医院儿研所干细胞研究室,儿童发育疾病研究教育部重点实验室,国家儿童健康与疾病临床医学研究中心,儿科学重庆市重点实验室,重庆 400014
- Keywords:
Chemokine C-X-C-motif receptor 7;
Hypoxia;
Urine-derived stem cell;
Cell proliferation;
Cell migration;
Real-time PCR
- From:
Acta Anatomica Sinica
2024;55(6):699-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhibition of chemokine C-X-C motif receptor 7(CXCR7)expression on the proliferation,migration,differentiation and mitochondrial function of human urine-derived stem cells(USCs)under hypoxia.Methods CXCR7 expression was inhibited by siCXCR7 and detected by Real-time PCR and Western blotting in hypoxia group treated with 3％O2 for 48 hours.Cell proliferation was detected by clonal formation assay and cell growth curve.Cell migration ability was detected by scratch assay and Transwell assay.Alkaline phosphatase,alizarin red,oil red O and alcian blue staining were used to detect the multidirectional differentiation ability of cells.Mitochondrial function was evaluated by JC-1 fluorescent probe,adenosine triphosphate(ATP)and reactive oxygen species(ROS).Results Compared with the normal oxygen group,the expression of CXCR7 in USCs in hypoxia group was significantly up-regulated,and hypoxia promoted the proliferation,migration and clonogenesis of USCs.SiCXCR7 inhibited the expression of CXCR7 and inhibited the effects of hypoxia on the proliferation,migration and clonogenesis of USCs,but had no effect on cell differentiation.Hypoxia treatment increased mitochondrial membrane potential and ATP levels,and decreased the production of reactive oxygen species,while CXCR7 inhibition decreased mitochondrial membrane potential and ATP production.Conclusion Hypoxia may enhance mitochondrial function of USCs through the CXCR7 signaling pathway,thereby promoting cell proliferation and migration.
