Non-structural maintenance of chromosome condensin Ⅰ complex subunit G promoting the proliferation,migration and invasion of ovarian cancer cells by the Akt signaling pathway
10.16098/j.issn.0529-1356.2024.05.008
- VernacularTitle:非染色体结构维护凝集素Ⅰ复合物亚基G通过Akt信号通路促进卵巢癌细胞的增殖、迁移和侵袭
- Author:
Ming-Shu ZHANG
1
;
Yi-Hui WANG
;
Qing ZHANG
;
Li-Ping YE
Author Information
1. 锦州医科大学基础医学院病理生理学教研室,辽宁锦州 121001;沈阳市第五人民医院医学检验中心,沈阳 110000
- Keywords:
Ovarian cancer;
Non-structural maintenonce of chromosome condensin Ⅰ complex subunit G;
Protein kinase B signaling pathway;
Proliferatiing cell nuclear antigen;
Matrix metallopeptidase 9;
Cadherin;
Western blotting
- From:
Acta Anatomica Sinica
2024;55(5):573-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory mechanism of non-structural maintenance of chromosome condensin Ⅰ complex submit G(NCAPG)on proliferation,migration,and invasion of ovarian cancer cells.Methods The bioinformatics database was used to analyze the differential expression of NCAPG in ovarian cancer tissues.Western blotting was used to detect the protein expression of NCAPG in normal ovarian epithelial cells IOSE80,ovarian cancer A2780 and SKOV3 cells.The silencing experiments of NCAPG siRNA were divided into blank,control,siNCAPG-1 and siNCAPG-2 groups.The overexpression experiments of NCAPG plasmid were divided into blank,control,NCAPG,NCAPG+MK2206 and MK2206 groups.MTT assay was used to detect cell proliferation activity.Cell scoring assay and transwell assay were used to analyze cell migration and invasion.The protein expressions of p-Akt,total(t)-Akt,proliferative cellular nucleus antigen(PCNA),matrix metallopeptidase 9(MMP-9),vimentin,N-cadherin,and E-cadherin were detected by Western blotting.Results NCAPG was highly expressed in ovarian cancer tissues and cells.Knockdown of NCAPG significantly inhibited the proliferation,invasion and migration of ovarian cancer SKOV3 cells.The protein expressions of p-Akt,PCNA,MMP-9,vimentin and N-cadherin decreased while E-cadherin expression increased.Overexpression of NCAPG significantly promoted the proliferation,invasion and migration of ovarian cancer A2780 cells.The protein expressions of p-Akt,PCNA,MMP-9,vimentin,and N-cadherin increased while E-cadherin expression decreased.Akt inhibitor MK2206 significantly attenuated the above effects of NCAPG.Conclusion NCAPG promotes the proliferation,invasion,and migration of ovarian cancer cells by activating the Akt signaling pathway and regulating the expression of PCNA,MMP-9,and epithelial-mesenchymal transition(EMT)-related proteins.
