Morphological features of the neural projection pathway from the dorsal raphe nucleus to the claustrum in mice
10.16098/j.issn.0529-1356.2024.04.006
- VernacularTitle:小鼠中缝背核向屏状核投射神经通路的形态学特征
- Author:
Tao GONG
1
;
Zhi-Ping CAI
;
Juan SHI
;
Yun-Qing LI
Author Information
1. 包头医学院人体解剖学教研室,内蒙古包头 014040;空军军医大学人体解剖学与组织学胚胎学教研室,梁銶琚脑研究中心,西安 710032
- Keywords:
Dorsal raphe nucleus;
Claustrum;
5-hydroxytryptamine ergic neuron;
Calcium ion/calmodulin dependent protein kinase Ⅱ positive neuron;
Nerve tract tracing;
Immunofluorescence;
Mouse
- From:
Acta Anatomica Sinica
2024;55(4):414-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the morphological features of the neural projection pathway from the dorsal raphe nucleus(DRN)to the claustrum(CLA)in mice.Methods After injection of the retrograde tracer 594 retrobeads into the right CLA(n=3)or the anterograde tracer biotinylated dextran amine(BDA)into the DRN(n=3),the distribution and the neurochemical feature of the retrogradely labeled neurons in the DRN,as well as the locations of nerve fibers and terminals throughout the brain and within the CLA,were observed by a combined immunofluorescent staining for 5-hydrozytryptamine(5-HT).The downstream target of the DRN-CLA projection was studied by injection of rabies retrograde monosynaptic virus(RV)into calcium ion/calmodulin dependent protein kinae Ⅱ(CaMK Ⅱ)-Cre or glutamate decarboxylase 67(GAD67)-Cre mice.Results Retrograde labeled neurons were observed in the rostral,middle,and caudal segments of the DRN,with those in the middle segment and in the ventral part of the nucleus(DRV)the densest.Over 90％of the retrograde labeled neurons were 5-HT positive.After injection of BDA into the DRN,dense fiber projections were observed in the ventral tegmental area,parafascicular nucleus,ventral pallidum,and central amygdala nucleus.In comparison,DRN derived fibers and terminals in the CLA were relatively sparse.After injection of RV trans-synaptic virus into the CLA of CaMK Ⅱ-Cre and GAD67-Cre mice,respectively,dense RV-labeled presynaptic neurons were observed in the DRN of CaMK Ⅱ-Cre mice,while those in the DRN of GAD67-Cre mice were rather sparse.Conclusion The DRN-CLA projection pathway is characterized by the dense 5-HTergic neurons in the DRV,the relatively sparse fibers or terminals in the CLA,and a primary synaptic connection with CaMK Ⅱ positive neurons in the CLA.
