Dexmedetomidine alleviates myocardial injury and inflammation in diabetic myocardial ischemia-reperfusion rats
10.16352/j.issn.1001-6325.2024.11.1538
- VernacularTitle:右美托咪定减轻糖尿病心肌缺血/再灌注大鼠心肌损伤及炎性反应
- Author:
Bin LIU
1
;
Wenping LIU
;
Tao JIN
Author Information
1. 河北省沧州中西医结合医院 麻醉科,河北 沧州 061000
- Keywords:
dexmetomidine;
miR-490-3p/FOXO1 axis;
myocardial ischemia/reperfusion in diabetes;
myocardial injury;
in-flammation
- From:
Basic & Clinical Medicine
2024;44(11):1538-1543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of dexmedetomidine(DEX)on myocardial injury and inflammation of rats with diabetic myocardial ischemia-reperfusion(MI/R).Methods The rats were divided into sham group,model grousp[The type 2 diabetes mellitus(T2DM)model was replicated by feeding high-fat and high-sugar combined with streptozotocin injection;MI/R injury model was replicated by coronary artery ligation],DEX group(T2DM model rats were injected with 10 μg/kg DEX through tail vein),antagomir NC group(T2DM model rats were injected with 10 μg/kg DEX and antagomir NC through tail vein),miR-490-3p antagomir group(T2DM model rats were injected with 10 μg/kg DEX and miR-490-3p antagomir via tail vein).RT-qPCR was applied to detect the expression of miR-490-3p and forkhead box O1(FOXO1)mRNA;Blood glucose meter was applied to measure fasting blood glucose(FBG)in rats;The level of fasting insulin(FINS),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH)and inflammatory factors interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)was measured by ELISA;HE staining microscopy was applied to observe pathological damage of myocar-dial tissue;TTC staining microscopy was applied to determine the size of myocardial infarction;Dual luciferase assay was applied to verify the relationship between miR-490-3p and FOXO1;Western blot was applied to detect the ex-pression of FOXO1 protein in myocardial tissue.Results Compared with the sham group,the FBG,FINS,CK-MB,LDH,IL-1β,TNF-α,myocardial infarction area,FOXO1 mRNA and protein expression in model group were all in-creased,while miR-490-3p expression decreased(P＜0.05);Compared with the model group,the FBG,FINS,CK-MB,LDH,IL-1β,TNF-α,myocardial infarction area,FOXO1 mRNA and protein expression in rats from DEX group decreased,the miR-490-3p expression increased(P＜0.05);Down regulation of miR-490-3p was able to signifi-cantly baffle the improvement of DEX on myocardial injury and inflammation in diabetes MI/R rats(P＜0.05);FOXO1 had a target-specific regulatory relationship with miR-490-3p.Conclusions DEX may inhibit inflammation and alleviate myocardial injury induced by MI/R in diabetic rat models by regulating the miR-490-3p/FOXO1 axis.
