Analysis of Fecal Non-Targeted Metabolomics in Patients with Chronic Aplastic Anemia and Its Correlation with Traditional Chinese Medicine Syndrome Types
10.13359/j.cnki.gzxbtcm.2024.11.002
- VernacularTitle:慢性再生障碍性贫血患者粪便非靶向代谢组学分析及与中医证型的相关性
- Author:
Lu-Qi CHEN
1
;
Jing-Jing XIE
;
Heng WANG
;
Jun LI
Author Information
1. 南京中医药大学附属医院,江苏 南京 210029
- Keywords:
chronic aplastic anemia;
gas chromatography-mass spectrometry(GC-MS);
non-targeted metabolomics;
traditional Chinese medicine syndrome types;
metabolic pathway
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(11):2831-2839
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the material base of traditional Chinese medicine(TCM)syndrome types of chronic aplastic anemia(CAA)by investigating the differences of fecal non-targeted metabolomics changes in patients with CAA and normal people,and by analyzing the differential fecal metabolite profiles in CAA patients with various TCM syndrome types.Methods The analysis was performed on 21 CAA patients(CAA group)who were initially treated at Affiliated Hospital of Nanjing University of Chinese Medicine from July 2018 to June 2021 and on 24 volunteers who had healthy medical checkup(normal group)during the same period.The 21 cases of CAA patients were categorized into four cases of kidney yang deficiency group,12 cases of kidney yin deficiency group,and five cases of deficiency of kidney yin and yang group according to the criteria of TCM syndrome differentiation and classification.Differential metabolites were screened after stool collection,gas chromatography-mass spectrometry(GC-MS)analysis and multivariate statistical analysis,and then enrichment analysis of the metabolic pathway was conducted.Results(1)Obvious differences were presented in 38 kinds of metabolites between the CAA group and the normal group,of which there were 27 metabolites,including 3-hydroxyphenylacetic acid,adenine,isocitric acid,L-glutamine,uric acid and guanine,having the up-regulated relative contents in CAA group,while there were 11 metabolites,including 3'-adenosine,eicosanoic acid,inosine,N-acetylornithine and norepinephrine,having the down-regulated relative contents in CAA group.The primary enriched metabolic pathways of the differential metabolites included arginine biosynthesis,purine metabolism,central carbon metabolism in cancer,glyoxylate and dicarboxylic acid metabolism,GABAergic synapses,metabolism of alanine,aspartate and glutamate,and D-glutamine and D-glutamate metabolism.(2)There were significant differences in 16 kinds of metabolites among the CAA patients with various TCM syndrome types,including 5-methoxytryptamine,epinephrine,arbutin,β-alanine,pentanediamine,inositol,pyruvate and tyramine,etc.The primary enriched metabolic pathways of the differential metabolites included neuroactive ligand-receptor interactions,tyrosine metabolism,phosphatidylinositol signaling system,pantothenic acid and coenzyme A biosynthesis,glycolysis/gluconeogenesis,primary bile acid biosynthesis,phosphoinositide metabolism,ascorbic acid and aldehyde acid metabolism,type Ⅱ diabetes mellitus,and adrenergic signal transduction in cardiomyocytes.Conclusion Significant differences exist in the fecal metabolomics between the CAA patients and the normal volunteers,which may reflect the metabolic changes of immune dysregulation and hematopoietic failure mediated by the altered intestinal flora in CAA patients.This study revealed the material base of CAA of various kidney deficiency syndromes for the first time at the level of fecal metabolomics,which provides a novel approach for the study of modernization of TCM syndrome.
