Expression and significance of ABCC4 and miR-125b-5p in prostate cancer tissue
10.3760/cma.j.cn115396-20231110-00136
- VernacularTitle:ABCC4和miR-125b-5p在前列腺癌组织中的表达及意义
- Author:
Jian GAO
1
;
Yuan ZHANG
;
Lei LI
Author Information
1. 秦皇岛市中医医院泌尿外科，秦皇岛 066000
- Keywords:
Prostatic neoplasms;
Carcinoma;
MicroRNAs;
ABC transporter family 4;
MiR-125b-5p
- From:
International Journal of Surgery
2024;51(6):382-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of ABC transporter family 4(ABCC4) and miR-125b-5p in prostate cancer tissues, and analyze their relationship with the prognosis of prostate cancer.Methods:From May 2019 to May 2021, 60 patients with prostate cancer diagnosed by pathological diagnosis admitted to Qinhuangdao Hospital of Traditional Chinese Medicine were selected. All fresh tissue samples of prostate cancer were obtained from prostate cancer surgery and divided into prostate cancer group (cancer tissue) and control group (adjacent tissue). Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of ABCC4 mRNA and miR-125b-5p in the cancer tissue and adjacent tissue. Immunohistochemistry was used to detect the expression of ABCC4 in paraffin sections of tissues, and to analyze the relationship between the expression levels of ABCC4 and miR-125b-5p and clinicopathological features, prognosis of patients with prostate cancer, and the influencing factors of poor prognosis of prostate cancer. The measurement data were expressed as mean ± standard deviation ( ± s), and t-test was used for comparison between groups; Chi-square test was used to compare the count data between groups. Pearson method was used to analyze the correlation between ABCC4 mRNA and miR-125b-5p levels in prostate cancer. Kaplan-Meier method was used to analyze the relationship between the expression levels of ABCC4 and miR-125b-5p and prognosis, and Log-rank test was used to compare the survival rates. Univariate and multivariate COX regression analysis was used to evaluate the influencing factors of poor prognosis of prostate cancer. Results:The results of RT-qPCR showed that the expression level of ABCC4 mRNA in prostate cancer group was higher than that in control group ( P<0.05), and the expression level of miR-125b-5p was lower than that in control group ( P<0.05). Immunohistochemistry showed that the positive rate of ABCC4 in prostate cancer tissues was higher than that in adjacent tissues ( P<0.05). Pearson correlation analysis showed that the expression level of ABCC4 mRNA was negatively correlated with miR-125b-5p ( r=-0.687, P<0.05). The expression level of ABCC4 and miR-125b-5p in patients with prostate cancer was related to tumor stage, serum prostate-specific antigen, Gleason score and tumor metastasis ( P<0.05). Kaplan-Meier analysis showed that the 3-year cumulative survival rate of ABCC4-positive patients was lower than that of negative patients ( P<0.05), and the 3-year cumulative survival rate of patients with high-expression of miR-125b-5p was higher than that of patients with low-expression ( P<0.05). Multivariate COX regression analysis showed that the low level of miR-125b-5p and the high level of ABC-C4 were the risk factors for the poor prognosis of prostate cancer ( HR=2.685, 3.461, P<0.05). Conclusion:The high-expression of ABCC4 and the low-expression of miR-125b-5p in prostate cancer tissues are related to many clinicopathological features and prognosis of prostate cancer, which are risk factors for poor prognosis of prostate cancer patients.
