Effects of stem cells combined with dual drug nanoassemblies on ventricular remodeling and cardiac function after acute myocardial infarction in rats
10.3760/cma.j.cn121382-20240221-00304
- VernacularTitle:干细胞联合双药纳米组装体对大鼠急性心肌梗死后心室重构和心功能的影响研究
- Author:
Xiaoxiao LIU
1
;
Chunfeng XIN
Author Information
1. 高唐县人民医院心内科，聊城 252800
- Keywords:
Stem cells;
Dual drug nanoassemblies;
Acute myocardial infarction;
Ventricular remodeling;
Cardiac function;
Angiotensin 1-7;
Temisal
- From:
International Journal of Biomedical Engineering
2024;47(3):227-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of stem cells combined with dual drug nanoassemblies on ventricular remodeling and cardiac function after acute myocardial infarction in rats.Methods:A dual drug nano assembly of angiotensin 1-7 (SAAl-7) peptide and temisal was constructed. Male SD-rats (100 cases) were randomly divided into the sham group, model group, nano group, stem cell group, and combination group. And each group had 20 cases. Acute myocardial infarction rat models were established with anterior descending artery ligation in the model group, nano group, stem cell group, and combination group. The rats in the sham group took the same surgical steps but did not ligate the ligation line. In addition, rats in the stem cell group and combination group were injected with 20 μl of stem cells at the edge of the infarct area after ligation during the surgery. Rats in the other groups were injected with equal doses of phosphate buffered solution (PBS) by the same method. Starting from the day of surgical modeling, rats in nano group and combined group were injected with 0.5 ml of two-drug nano assemblies in the tail vein, while rats in the sham group, model group, stem cell group received a tail vein injection of an equal volume of 0.9% sodium chloride solution. After surgery, rats in all groups were continuously observed for 14 days. The cardiac function indicators, ventricular remodeling indicators, serum indicators, the myocardial infarction area, and the myocardial cell apoptosis rate of rats in all groups were detected and compared. The cardiac function indicators include left ventricular ejection fraction (LVEF), fraction shorting (FS), left ventricular end diastolic diameter (LVEDD), and left ventricular end-systolic diameter (LVESD). The ventricular remodeling indicators include left ventricle weight (LVW), left ventricular mass index (LVWI), and transdiameter of cardiomyocytes (TDM). The serum indicators include interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD), glutathione, malondialdehyde, creatinine, and alanine aminotransferase (ALT).Results:Compared with the sham group, LVEF, FS, body mass, SOD, and glutathione levels in the model group were decreased (all P < 0.05), while LVEDD, LVESD, LVW, LVWI, TDM, IL-6, TNF-α, malondialdehyde, creatinine, ALT, myocardial infarction area, and myocardial cell apoptosis rate were increased (all P < 0.05). Compared with the model group, LVEF, FS, body mass, SOD, and glutathione levels in the nano group, stem cell group, and combination group were increased (all P < 0.05), while LVEDD, LVESD, LVW, LVWI, TDM, IL-6, TNF-α, malondialdehyde, creatinine, ALT, myocardial infarction area, and myocardial cell apoptosis rate were decreased (all P < 0.05). Compared with the nano group and stem cell group, LVEF, FS, body mass, SOD, and glutathione levels in the combination group were increased (all P < 0.05), while LVEDD, LVESD, LVW, LVWI, TDM, IL-6, TNF-α, malondialdehyde, creatinine, ALT, myocardial infarction area, and myocardial cell apoptosis rate were decreased (all P < 0.05). Conclusions:The combination of stem cells and dual drug nano assemblies can effectively treat acute myocardial infarction rats, improve their cardiac function, and reduce ventricular remodeling, which may be related to the reducing inflammatory response and oxidative stress inhibiting myocardial cell apoptosis and narrowing the area of myocardial infarction.
