Correlation between peripheral blood miR-34a,miR-431,and miR-183 levels with hemodynamics and hearing prognosis in patients with sudden deafness
10.3969/j.issn.1673-4130.2024.22.007
- VernacularTitle:外周血miR-34a、miR-431、miR-183水平与突发性耳聋患者血流动力学及听力预后的相关性
- Author:
Jinjin SHEN
1
;
Hongyan HU
;
Min XU
Author Information
1. 空军军医大学第一附属医院耳鼻喉科,陕西西安 710032
- Keywords:
sudden deafness;
microRNA-34a;
microRNA-431;
microRNA-183;
hemodynamics;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(22):2721-2725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between peripheral blood microRNA-34a(miR-34a),mi-croRNA-431(miR-431),and microRNA-183(miR-183)levels with hemodynamics and hearing prognosis in patients with sudden deafness(SD).Methods A total of 132 patients with SD who visited the First Affiliated Hospital of Air Force Medical University(the hospital)from January 2021 to December 2023 were included as the disease group,132 healthy individuals(without SD)who came to the hospital for physical examination were used as the control group.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the levels of miR-34a,miR-431,and miR-183 in peripheral blood.Pearson correlation was applied to analyze the correlation between peripheral blood miR-34a,miR-431,miR-183 levels and hemodynamic indicators.Multiple Logistic regression analysis(stepwise forward method)was applied to screen for factors affecting the hearing prognosis of patients with SD.Receiver operating characteristic(ROC)curve was plotted to obtain the area under the curve(AUC)of the single and combination of peripheral blood miR-34a,miR-431,and miR-183 in predicting hearing prognosis in patients with SD,and the AUC was compared using Z-test.Results The levels of miR-34a and miR-431 in the peripheral blood in the disease group were greatly higher than those in the con-trol group,while the level of miR-183 was greatly lower than that in the control group(P＜0.05).After treatment,the whole blood high shear viscosity(HSV),whole blood low shear viscosity(LSV),plasma vis-cosity(PV)of patients with SD were greatly lower than those before treatment(P＜0.05).The levels of miR-34a and miR-431 in peripheral blood of patients with SD were positively correlated with pre-treatment levels of HSV,LSV,and PV(P＜0.05),while the levels of miR-183 were negatively correlated with pre-treatment levels of HSV,LSV,and PV(P＜0.05).The miR-34a and miR-431 levels in the peripheral blood in the good prognosis group were greatly lower than those in the poor prognosis group,and the miR-183 level was greatly higher than that in the poor prognosis group(P＜0.05).The risk factors affecting the hearing prognosis of patients with SD included miR-34a and miR-431,and miR-183 was a protective factor affecting the hearing prognosis of patients with SD(P＜0.05).The AUC of peripheral blood miR-34a,miR-431,and miR-183 in predicting hearing prognosis in patients with SD was 0.969(95％CI:0.938-1.00),and the pre-dictive value of the the combination of the three was higher than that of miR-34a(Z=2.336,P=0.019),miR-431(Z=2.157,P=0.031),and miR-183(Z=2.351,P=0.019)alone.Conclusion The levels of miR-34a and miR-431 are abnormally elevated in peripheral blood of patients with SD,and are positively correlated with hemodynamic indicators.The level of miR-183 is abnormally reduced and is negatively correlated with hemodynamic indicators.The combination of the three has certain predictive value for the hearing prognosis of patients with SD.
