Expression and significance of lncRNA CCAT2 and NDRG1 in colorectal cancer tissues
10.3969/j.issn.1673-4130.2024.20.002
- VernacularTitle:结直肠癌组织中lncRNA CCAT2和NDRG1的表达及意义
- Author:
Yujie ZHOU
1
;
Fang YANG
;
Jing YAN
;
Zheng QIAN
Author Information
1. 南通市中医院肛肠科,江苏南通 226001
- Keywords:
colorectal cancer;
long non-coding RNA colon cancer associated transcript 2;
N-myc down-stream regulated gene 1;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(20):2437-2442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of long non-coding RNA(lncRNA)colon cancer associ-ated transcript 2(CCAT2)and N-myc downstream regulated gene(NDRG)1 in colorectal cancer(CRC)tis-sues and their relationship with clinicopathological features and prognosis.Methods A total of 96 patients with CRC who underwent radical surgery in Nantong Hospital of Traditional Chinese Medicine from February 2018 to February 2020 were selected as the research objects.Quantitative real-time PCR was used to detect the mRNA expression of lncRNA CCAT2 and NDRG1 in tissues.The expression of NDRG1 protein was detected by immunohistochemistry.Kaplan-Meier curve was used to analyze the prognosis differences of patients with different lncRNA CCAT2 and NDRG1 mRNA expression groups.Multivariate Cox regression was used to an-alyze the prognostic factors of CRC.Results The expression of lncRNA CCAT2 in cancer tissues was higher than that in adjacent tissues,and the expression of NDRG1 mRNA and protein was lower than that in adjacent tissues(P＜0.05).The expression of lncRNA CCAT2 was higher and the expression of NDRG1 mRNA was lower in TNM stage Ⅲ CRC patients with lymph node metastasis than in TNM stage Ⅰ-Ⅱ CRC patients without lymph node metastasis(P＜0.05).The 3-year cumulative survival rate of lncRNA CCAT2 high ex-pression group was lower than that of lncRNA CCAT2 low expression group,while the 3-year cumulative sur-vival rate of NDRG1 mRNA high expression group was higher than that of NDRG1 mRNA low expression group,and the difference was statistically significant(P＜0.05).TNM stage,lymph node metastasis,lncRNA CCAT2 and NDRG1 mRNA were the prognostic factors of CRC(P＜0.05).Conclusion The expression of lncRNA CCAT2 is increased and the expression of NDRG1 is decreased in CRC tissues.Both lncrna CCAT2 and NDRG1 are involved in the progression of CRC and can be used as new indicators for evaluating the sur-vival and prognosis of CRC patients.
