Expression changes of RUNX3 and J AM-A in cancer tissues of patients with triple-negative breast cancer and their correlation with disease prognosis
10.3969/j.issn.1673-4130.2024.16.013
- VernacularTitle:三阴性乳腺癌患者癌组织中RUNX3及JAM-A表达变化及与疾病预后的相关性分析
- Author:
Xue HAN
1
;
Shuai LI
;
Wenjing MA
;
Changmin WANG
Author Information
1. 新疆维吾尔自治区人民医院临床检验中心,新疆乌鲁木齐 830001
- Keywords:
triple-negative breast cancer;
Runt-related transcription factor 3;
junction adhesion mole-cule A;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(16):1986-1990,1995
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression changes of Runt-related transcription factor 3(RUNX3)and junction adhesion molecule A(JAM-A)in cancer tissues of patients with triple-negative breast cancer and their correlation with disease prognosis.Methods Sixty patients with triple-negative breast cancer diagnosed in the People's Hospital of Xinjiang Uygur Autonomous Region from January 2016 to March 2018 were select-ed as the study objects.Breast cancer tissues and adjacent tissues of patients were collected.Real-time fluores-cence quantitative PCR and enzyme-linked immunosorbent assay were used to detect and compare RUNX3 and JAM-A transcription levels and protein levels in cancer tissues and adjacent tissues.The transcription and pro-tein levels of RUNX3 and JAM-A in cancer tissues of patients with triple-negative breast cancer with different clinicopathological characteristics were compared.The correlation between RUNX3 and JAM-A expression and poor prognosis in patients with triple-negative breast cancer was analyzed by Spearman correlation analy-sis.Results The transcription level of RUNX3 in cancer tissues was significantly lower than that in adjacent tissues,and the transcription level of JAM-A was significantly higher than that in adjacent tissues,with statis-tical significance(P＜0.05).The level of RUNX3 protein in cancer tissues of patients with triple-negative breast cancer was significantly lower than that in adjacent tissues,and the level of JAM-A protein was signifi-cantly higher than that in adjacent tissues,with statistical significance(P＜0.05).There were statistically sig-nificant differences in RUNX3 and JAM-A transcription levels in triple-negative breast cancer patients with different tumor maximum diameter,TNM stage and lymph node metastasis(P＜0.05).There were statisti-cally significant differences in RUNX3 and JAM-A protein levels among patients with triple-negative breast cancer with different tumor maximum diameter,TNM stage and lymph node metastasis(P＜0.05).Spearman correlation analysis showed that RUNX3 transcription and protein levels were negatively correlated with poor prognosis,while JAM-A transcription and protein levels were positively correlated with poor prognosis in pa-tients with triple-negative breast cancer(P＜0.05).Conclusion RUNX3 expression is significantly down-regulated and JAM-A expression is significantly up-regulated in cancer tissues of patients with triple-negative breast cancer,and there is a certain correlation with patient prognosis.RUNX3 and JAM-A may be important regulatory factors in the occurrence and development of triple-negative breast cancer.
